SBF SEM images of a Zebrafish hindbrain containing several Toxoplasma gondii tachizoites at different stages of replication

REL 2020-07-03 2021-10-27 2022-02-08 SBF SEM images of a Zebrafish hindbrain containing several Toxoplasma gondii tachizoites at different stages of replication 0000-0002-5703-2922 Marie-Charlotte Domart Francis Crick Institute, Electron Microscopy STP 1 Midland Road London London United Kingdom NW1 1AT 0000-0002-5703-2922 Marie-Charlotte Domart Francis Crick Institute, Electron Microscopy STP 1 Midland Road London London United Kingdom NW1 1AT Peddie CJ Domart MC Collinson L 553.9 10.6019/EMPIAR-10464 CLEM Yoshida N Domart MC Peddie CJ Yakimovich A Mazon-Moya MJ Hawkins TA Collinson L Mercer J Frickel EM Mostowy S The zebrafish as a novel model for the <i>in vivo</i> study of <i>Toxoplasma gondii</i> replication and interaction with macrophages Disease models & mechanisms Dis Model Mech 7 13 2020 English 10.1242/dmm.043091 32461265

Raw data corresponding to Suppl. Fig 2, Suppl. Fig 4 and Suppl. Fig. 6 in the paper

S-BSST429 Survey SBF SEM images showing the position of the imaged ROIs at the start of each run (3 runs- #20-22) /data/Survey_images micrographs - single frame DM4 DM4 3 1 UNSIGNED 16 BIT INTEGER variable variable variable variable

Survey images showing the position of the ROIs imaged for each run, if opened in Digital Micrograph. Variable pixel widths and heights. Run 20: 5000 x 5000, run 21-22: 4096 x 4096. Variable pixel widths. Run 20: 75.6nm, run 21: 87.2nm, run 22: 86.3nm

Low magnification raw SBF SEM overview images (ROI00) of Toxoplasma Gondii tachizoites in a Zebrafish hindbrain over 3 runs (20-22) /data/P400_SBF_SEM_F1S1_run20-22_EMPIAR-10464/ROI00 micrographs - single frame DM4 DM4 1099 1 UNSIGNED 16 BIT INTEGER variable variable variable variable

Low magnification overview SBF SEM images (ROI00) for runs 20-22, linked to higher magnification images in ROI01 and ROI02. Imaged at 2kV, 2us dwell, variable pressure 5Pa. Variable pixel widths and heights. Run 20: 7500 x 5497, run 21: 7000 x 4000, run 22: 6000 x 4000. Variable pixel widths. Run 20: 48.5nm, run 21: 54nm, run 22: 49nm.

High magnification raw SBF SEM images (ROI01) of Toxoplasma Gondii tachizoites in a Zebrafish hindbrain over 3 runs (20-22) /data/P400_SBF_SEM_F1S1_run20-22_EMPIAR-10464/ROI01 micrographs - single frame DM4 DM4 1099 1 UNSIGNED 16 BIT INTEGER variable variable variable variable

High magnification images (ROI01) linked to lower magnification images in ROI00, for 3 runs (20-22). Imaged at 2kV, 2us dwell, variable pressure 5Pa. Variable pixel widths and heights. Run 20: 16384 x 16384, run 21: 15000 x 10000, run 22: 15000 x 12000. Variable pixel widths. Run 20: 6.6nm, run 21-22: 5.8nm.

High magnification raw SBF SEM images (ROI02) of Toxoplasma Gondii tachizoites in a Zebrafish hindbrain (run 20) /data/P400_SBF_SEM_F1S1_run20-22_EMPIAR-10464/run20_ROI02 micrographs - single frame DM4 DM4 391 1 UNSIGNED 16 BIT INTEGER 8192 69.0 8192 69.0

High magnification images (ROI02) linked to lower magnification images in ROI00 for run 20. Imaged at 2kV, 2us dwell, variable pressure 5Pa.

High magnification raw SBF SEM images (ROI02) of Toxoplasma Gondii tachizoites in a Zebrafish hindbrain for runs 21-22 /data/P400_SBF_SEM_F1S1_run20-22_EMPIAR-10464/run21-22_ROI02 micrographs - single frame DM4 DM4 705 1 UNSIGNED 16 BIT INTEGER variable variable variable variable

High magnification images (ROI02) linked to lower magnification images in ROI00 for run 21-22. Note that it is a different ROI than ROI02-run 20. Imaged at 2kV, 2us dwell, variable pressure 5Pa. Variable pixel widths and heights. Run 21: 4000 x 4000, run 22: 5000 x 5000. Variable pixel widths. Run 21: 7.1nm, run 22: 6.2nm.