SBF SEM images of a Zebrafish hindbrain containing several Toxoplasma gondii tachizoites at different stages of replication

REL 2020-07-03 2020-12-09 2022-02-08 SBF SEM images of a Zebrafish hindbrain containing several Toxoplasma gondii tachizoites at different stages of replication 0000-0002-5703-2922 Marie-Charlotte Domart Francis Crick Institute, Electron Microscopy STP 1 Midland Road London London United Kingdom NW1 1AT 0000-0002-5703-2922 Marie-Charlotte Domart Francis Crick Institute, Electron Microscopy STP 1 Midland Road London London United Kingdom NW1 1AT Peddie CJ Domart MC Collinson L 175.2 10.6019/EMPIAR-10463 CLEM Yoshida N Domart MC Peddie CJ Yakimovich A Mazon-Moya MJ Hawkins TA Collinson L Mercer J Frickel EM Mostowy S The zebrafish as a novel model for the <i>in vivo</i> study of <i>Toxoplasma gondii</i> replication and interaction with macrophages Disease models & mechanisms Dis Model Mech 7 13 2020 English 10.1242/dmm.043091 32461265

Raw data corresponding to Fig. 2 and Suppl. Fig 2 in the paper

S-BSST429 Survey SBF SEM image showing the position of the 5 imaged ROIs at the start of the run /data micrographs - single frame DM4 DM4 1 1 UNSIGNED 16 BIT INTEGER 5000 742.5 5000 742.5

Survey image showing the position of the 5 ROIs imaged (ROI00, 01-4) if opened in Digital Micrograph

Low magnification raw SBF SEM overview images (ROI00) of Toxoplasma Gondii tachizoites in a Zebrafish hindbrain /data/P400_SBF_SEM_F1S1_run17_EMPIAR-10463/ROI_00 micrographs - single frame DM4 DM4 354 1 UNSIGNED 16 BIT INTEGER 6500 504.0 5000 504.0

Low magnification overview images (ROI00) linked to higher magnification images in ROI01. Imaged at 2kV, 2us dwell, variable pressure 5Pa.

High magnification raw SBF SEM images (ROI01) of Toxoplasma Gondii tachizoites in a Zebrafish hindbrain /data/P400_SBF_SEM_F1S1_run17_EMPIAR-10463/ROI_01 micrographs - single frame DM4 DM4 354 1 UNSIGNED 16 BIT INTEGER 15000 60.0 8000 60.0

High magnification images (ROI01) linked to lower magnification images in ROI00. Imaged at 2kV, 2us dwell, variable pressure 5Pa.

High magnification raw SBF SEM images (ROI02) of Toxoplasma Gondii tachizoites in a Zebrafish hindbrain /data/P400_SBF_SEM_F1S1_run17_EMPIAR-10463/ROI_02 micrographs - single frame DM4 DM4 354 1 UNSIGNED 16 BIT INTEGER 8000 64.0 8000 64.0

High magnification images (ROI02) linked to lower magnification images in ROI00. Imaged at 2kV, 2us dwell, variable pressure 5Pa.

High magnification raw SBF SEM images (ROI03) of Toxoplasma Gondii tachizoites in a Zebrafish hindbrain /data/P400_SBF_SEM_F1S1_run17_EMPIAR-10463/ROI_03 micrographs - single frame DM4 DM4 354 1 UNSIGNED 16 BIT INTEGER 4000 64.0 8000 64.0

High magnification images (ROI03) linked to lower magnification images in ROI00. Imaged at 2kV, 2us dwell, variable pressure 5Pa.

High magnification raw SBF SEM images (ROI04) of Toxoplasma Gondii tachizoites in a Zebrafish hindbrain /data/P400_SBF_SEM_F1S1_run17_EMPIAR-10463/ROI_04 micrographs - single frame DM4 DM4 354 1 UNSIGNED 16 BIT INTEGER 3000 78.0 3000 78.0

High magnification images (ROI04) linked to lower magnification images in ROI00. Imaged at 2kV, 2us dwell, variable pressure 5Pa.

Low magnification aligned SBF SEM images (ROI00) with segmentation of middle section of Toxoplasma Gondii tachizoites in a Zebrafish hindbrain /data/ROI00_composite micrographs - multiframe TIFF TIFF 354 2 UNSIGNED 16 BIT INTEGER 3025 500.0 2326 500.0

Low magnification aligned (least square in TrakEM2) overview SBF SEM images (ROI00) and manual segmentation (channel 2) of middle sections of Toxoplasma tachizoites. Used for correlation with fish 1 (see Biostudies link). See Fig 2 of the paper.