<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10452" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2020-06-22</depositionDate>
            <releaseDate>2020-08-18</releaseDate>
            <updateDate>2020-08-18</updateDate>
        </keyDates>
        <title>Cryo Electron Tomograms of Membrane Fractions of Rabbit Skeletal Muscle for Structural Determination of RyR1 in SR Vesicles at subnanometer resolution</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0003-3550-6274</authorORCID>
            <firstName>Misha</firstName>
            <lastName>Kudryashev</lastName>
            <organization type="academic">Max Planck  Institute for Biophysics</organization>
            <street>Max von Laue Strasse 3</street>
            <townOrCity>Frankfurt am Main</townOrCity>
            <country>Germany</country>
            <postOrZipCode>60438</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-3550-6274</authorORCID>
            <firstName>Misha</firstName>
            <lastName>Kudryashev</lastName>
            <organization type="academic">Max Planck  Institute for Biophysics</organization>
            <street>Max von Laue Strasse 3</street>
            <townOrCity>Frankfurt am Main</townOrCity>
            <country>Germany</country>
            <postOrZipCode>60438</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-2854-0119">Sanchez RM</author>
            <author authorORCID="0000-0002-6478-476X">Zhang Y</author>
            <author authorORCID="0000-0001-5730-5567">Chen W</author>
            <author authorORCID="0000-0002-9661-4554">Dietrich L</author>
            <author authorORCID="0000-0003-3550-6274">Kudryashev M</author>
        </authorsList>
        <datasetSize units="GB">435.8</datasetSize>
        <entryDOI>10.6019/EMPIAR-10452</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-10840</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-2854-0119" order="1">Sanchez RM</author>
                    <author authorORCID="0000-0002-6478-476X" order="2">Zhang Y</author>
                    <author authorORCID="0000-0001-5730-5567" order="3">Chen W</author>
                    <author authorORCID="0000-0002-9661-4554" order="4">Dietrich L</author>
                    <author authorORCID="0000-0003-3550-6274" order="5">Kudryashev M</author>
                    <title>Subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM</title>
                    <journal>Nature communications</journal>
                    <journalAbbreviation>Nat Commun</journalAbbreviation>
                    <country>Germany</country>
                    <issue>1</issue>
                    <volume>11</volume>
                    <year>2020</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41467-020-17466-0</externalReferences>
                    <externalReferences type="pubmed">32709843</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>48 tilt series (frames aligned my motioncorr2) in .st stacks (mrc format for imod) including .tlt, .xf and .defocus files to generate reconstructions</name>
        <directory>/data</directory>
        <category>tilt series</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>48</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>3710</imageWidth>
            <pixelWidth>1.7</pixelWidth>
            <imageHeight>3838</imageHeight>
            <pixelHeight>1.7</pixelHeight>
        </dimensions>
        <details>The alignment parameters .tlt and .xf files could be used to perform 3D reconstruction using Imod; the locations of the particles and the alignment parameters are located in the .tbl file (Dynamo-style). Column 23 in the table indicates the separation into independent half-sets.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
