REL
2020-06-23
2020-12-11
2020-12-11
Cryo electron tomography after FIB-milling of Planctomycetes species Tuwongella immobilis (33k magnification)
0000-0003-0241-9158
Christian
Seeger
Department of Cell and Molecular Biology, Molecular Evolution, Uppsala University
Uppsala
Sweden
75124
0000-0003-0241-9158
Siv
Andersson
Department of Cell and Molecular Biology
Husargatan 3
Uppsala
Uppsala
Sweden
75124
Seeger C
Andersson SG
15.5
10.6019/EMPIAR-10451
EMDB
EMD-11124
Mahajan M
Seeger C
Yee B
Andersson SGE
Evolutionary Remodeling of the Cell Envelope in Bacteria of the Planctomycetes Phylum
Genome biology and evolution
Genome Biol Evol
Sweden
9
12
1528
1548
2020
English
10.1093/gbe/evaa159
32761170
Tilt series obtained during cryo-ET data collection using SerialEM on Titan Krios; species: Tuwongella immobilis (33k magnification); number of tilts: 61; total dose of tilt series: 100e-/Å^2
Single micrographs from cryo-ET tilt series (uncorrected) of Tuwongella immobilis after FIB-milling (33k magnification)
/data/micrographs of tilt series (uncorrected)
micrographs - single frame
MRC
MRC
61
5
32 BIT FLOAT
3710
4.291
3838
4.291
Uncorrected micrographs from cryo-ET tilt series from Tuwongella immobilis obtained after FIB-milling. Total electron dose: 95e-/Å^2; 61 tilts; -60°/+60°, 2° increment, start at +20°; defocus: -4µm
data/micrographs of tilt series (uncorrected)/CS_04_grid2_tomo_001_000_20.0.mrc
data/micrographs of tilt series (uncorrected)/CS_04_grid2_tomo_001_000_20.0.mrc
data/micrographs of tilt series (uncorrected)
Single micrographs from cryo-ET tilt series (motion-corrected) of Tuwongella immobilis after FIB-milling (33k magnification)
/data/micrographs of tilt series (motion corrected)
micrographs - single frame
MRC
MRC
61
1
SIGNED 16 BIT INTEGER
3710
4.291
3838
4.291
Motion-corrected micrographs from cryo-ET tilt series from Tuwongella immobilis obtained after FIB-milling. Total electron dose: 95e-/Å^2; 61 tilts; -60°/+60°, 2° increment, start at +20°; defocus: -4µm
data/micrographs of tilt series (motion corrected)/CS_04_grid2_tomo_001_000_20.0_MC.mrc
data/micrographs of tilt series (motion corrected)/CS_04_grid2_tomo_001_000_20.0_MC.mrc
data/micrographs of tilt series (motion corrected)
Image stack (motion-corrected) from cryo-ET tilt series of Tuwongella immobilis after FIB-milling (33k magnification)
/data/stacked tilt series (motion corrected)
tilt series
MRC
MRC
61
1
32 BIT FLOAT
3710
4.291
3838
4.291
Image stack (motion corrected) from cryo-ET tilt series from Tuwongella immobilis obtained after FIB-milling. Total electron dose: 95e-/Å^2; 61 tilts; -60°/+60°, 2° increment, start at +20°; defocus: -4µm