REL 2020-06-04 2020-10-23 2020-10-23 0000-0003-2190-4909 Ilya Belevich Electron Microscopy Unit, Institute of Biotechnology, University of Helsinki PL56 (Viikinkaari 9) Helsinki Uusimaa Finland 00014 0000-0002-7287-8459 Yrjö Helariutta Sainsbury Laboratory, University of Cambridge Bateman Street Cambridge Cambridgeshire United Kingdom CB2 1LR Paterlini A Belevich I Jokitalo E Helariutta Y 43.2 10.6019/EMPIAR-10442 SBF-SEM Yan D Yadav SR Paterlini A Nicolas WJ Petit JD Brocard L Belevich I Grison MS Vaten A Karami L El-Showk S Lee JY Murawska GM Mortimer J Knoblauch M Jokitalo E Markham JE Bayer EM Helariutta Y Nature plants Nat Plants 6 5 604 615 2019 English 10.1038/s41477-019-0429-5 31182845 Sieve element cells of Arabidopsis thaliana roots /data/MRC_Files micrographs - multiframe MRC MRC 1 1061 1 1061 UNSIGNED BYTE 3874 70.0 3253 70.0

Processed datasets: image processing was done in Microscopy Image Browser and included adjustment of contrast, alignment and calibration. The bounding box coordinates represent position of the dataset relative to the tip of the root Dataset: 170314_Col-0_R20_339-381um.am Voxel size: 0.007 x 0.007 x 0.04 um BoundingBox (xmin, xmax, ymin, ymax, zmin, zmax) 527.30140 555.40970 -686.83160 -663.23020 339.14530 379.14530 MIB(1704051124): Flip: mode=flipZ MIB(1704051130): ContrastGamma: Min:12585.2355, Max: 36949.7536, Gamma: 1 MIB(1704051130): Converted to from uint16 to uint8 MIB(1704051135): Aligned using Drift correction; relative to 0 MIB(1704051143): Aligned using Single landmark point; relative to 0 MIB(1704051206): ImCrop: [x1 y1 dx dy z1 dz t1 dt]: [682 418 3874 3253 1 1061 1 1]

Sieve element cells of Arabidopsis thaliana roots /data/MRC_Files micrographs - multiframe MRC MRC 1 544 1 544 UNSIGNED BYTE 3430 70.0 2010 70.0

Processed datasets: image processing was done in Microscopy Image Browser and included adjustment of contrast, alignment and calibration. The bounding box coordinates represent position of the dataset relative to the tip of the root Dataset: 170821_Col-0_R01_294-317um.am Voxel size: 0.007 x 0.007 x 0.04 um BoundingBox -46.17730 -20.82255 71.14690 86.00187 294.89500 314.89500 MIB(1709011612): Aligned using Single landmark point; relative to 0 MIB(1709011614): Aligned using Drift correction; relative to 0 MIB(1709011623): Aligned using Single landmark point; relative to 0 MIB(1709011623): ImCrop: [x1 y1 dx dy z1 dz t1 dt]: [119 482 3430 2010 1 544 1 1] MIB(1709011626): Normalize contrast, mode: mask, Ch: 1 MIB(1709011628): ContrastGamma: Min:14265.0396, Max: 23898.9022, Gamma: 1 MIB(1709011628): Converted to from uint16 to uint8 MIB(1709011628): Flip: mode=flipZ

Sieve element cells of Arabidopsis thaliana roots /data/MRC_Files micrographs - multiframe MRC MRC 1 717 1 717 UNSIGNED BYTE 2341 100.0 1689 100.0

Processed datasets: image processing was done in Microscopy Image Browser and included adjustment of contrast, alignment and calibration. The bounding box coordinates represent position of the dataset relative to the tip of the root Dataset: 180130_PLM_SE_down_278-307um.am Voxel size: 0.010 x 0.01 x 0.04 um BoundingBox -166.42400 -143.02300 -290.90100 -274.02000 278.23500 306.23500 MIB(1802021325): Aligned using Drift correction; relative to 0 MIB(1802021327): Aligned using Single landmark point; relative to 0 MIB(1802021330): Normalize contrast, mode: mask, Ch: 1 MIB(1802021334): Normalize contrast, mode: mask, Ch: 1 MIB(1802021335): ContrastGamma: Min:29058.337, Max: 44837.221, Gamma: 1 MIB(1802021335): Converted to from uint16 to uint8 MIB(1802021336): ImCrop: [x1 y1 dx dy z1 dz t1 dt]: [296 448 2341 1689 1 717 1 1]

Sieve element cells of Arabidopsis thaliana roots /data/MRC_Files micrographs - multiframe MRC MRC 1 717 1 717 UNSIGNED BYTE 2596 100.0 1731 100.0

Processed datasets: image processing was done in Microscopy Image Browser and included adjustment of contrast, alignment and calibration. The bounding box coordinates represent position of the dataset relative to the tip of the root Dataset: 180130_PLM_SE_up_278-307um.am Voxel size: 0.010 x 0.01 x 0.04 um BoundingBox -161.23100 -135.28000 -327.25700 -309.95700 278.23500 306.23500 MIB(1802021310): Aligned using Drift correction; relative to 0 MIB(1802021312): Aligned using Single landmark point; relative to 0 MIB(1802021316): ImCrop: [x1 y1 dx dy z1 dz t1 dt]: [183 462 2596 1731 1 717 1 1] MIB(1802021319): Normalize contrast, mode: mask, Ch: 1 MIB(1802021320): ContrastGamma: Min:28724.6413, Max: 45826.6123, Gamma: 1 MIB(1802021320): Converted to from uint16 to uint8