<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10439" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2020-06-03</depositionDate>
            <releaseDate>2020-06-19</releaseDate>
            <updateDate>2022-01-31</updateDate>
        </keyDates>
        <title>SARS-CoV-2 ORF3a dimer in an MSP1E3D1 lipid nanodisc</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-6768-3406</authorORCID>
            <firstName>Stephen</firstName>
            <middleName>Graf</middleName>
            <lastName>Brohawn</lastName>
            <organization type="academic">UC Berkeley</organization>
            <townOrCity>Berkeley</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94720</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-6768-3406</authorORCID>
            <firstName>Stephen</firstName>
            <middleName>Graf</middleName>
            <lastName>Brohawn</lastName>
            <organization type="academic">UC Berkeley</organization>
            <townOrCity>Berkeley</townOrCity>
            <stateOrProvince>California</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>94720</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0001-8529-9045">Kern DM</author>
            <author authorORCID="0000-0001-6742-1094">Sorum B</author>
            <author>Mali SS</author>
            <author authorORCID="0000-0002-1344-0780">Hoel CM</author>
            <author>Sridharan S</author>
            <author>Remis JP</author>
            <author>Toso DB</author>
            <author>Kotecha A</author>
            <author>Bautista DM</author>
            <author authorORCID="0000-0001-6768-3406">Brohawn SG</author>
        </authorsList>
        <datasetSize units="TB">4.4</datasetSize>
        <entryDOI>10.6019/EMPIAR-10439</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-22136</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="true">
                    <author authorORCID="0000-0001-8529-9045" order="1">Kern DM</author>
                    <author authorORCID="0000-0001-6742-1094" order="2">Sorum B</author>
                    <author order="3">Mali SS</author>
                    <author authorORCID="0000-0002-1344-0780" order="4">Hoel CM</author>
                    <author order="5">Sridharan S</author>
                    <author order="6">Remis JP</author>
                    <author order="7">Toso DB</author>
                    <author order="8">Kotecha A</author>
                    <author order="9">Bautista DM</author>
                    <author authorORCID="0000-0001-6768-3406" order="10">Brohawn SG</author>
                    <title>Cryo-EM structure of the SARS-CoV-2 3a ion channel in lipid nanodiscs</title>
                    <journal>bioRxiv</journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country></country>
                    <year>2020</year>
                    <externalReferences type="doi">10.1101/2020.06.17.156554</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame movies of SARS-CoV-2 ORF3a dimer in an MSP1E3D1 lipid nanodisc</name>
        <directory>/data/frames</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>6309</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.5685</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.5685</pixelHeight>
        </dimensions>
        <details>Gain reference is in frames folder. Dose per frame for movies in "frames" is  1.00044 e/A2 and dose per frame for movies in "frames2" folder is 1.0135 e/A2. The final polished particles are in "3a-for-empiar". Movies were taken on a Talos Arctica with K3 camera.</details>
        <segmentationList/>
        <micrographsFilePattern>data/3a-for-empiar/final-particles.mrcs</micrographsFilePattern>
        <pickedParticlesFilePattern>data/3a-for-empiar/final-particles.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/3a-for-empiar</pickedParticlesDirectory>
    </imageSet>
</entry>
