REL 2020-06-10 2020-08-12 2020-08-12 0000-0001-7097-348X Maria Leptin European Molecular Biology Laboratory Meyerhofstrasse 1 Heidelberg BW Germany 69117 0000-0001-7097-348X Maria Leptin European Molecular Biology Laboratory Meyerhofstrasse 1 Heidelberg BW Germany 69117 0000-0001-8027-1836 Yannick Schwab EMBL Meyerhofstrasse 1 Heidelberg BW Germany 69117 Mathew R Rios-Barrera LD Machado P Schwab Y Leptin M 366.7 10.6019/EMPIAR-10434 SBF-SEM Mathew R Rios-Barrera LD Machado P Schwab Y Leptin M The EMBO journal EMBO J. United States 2020 English 10.15252/embj.2020105332 32657472

The micrographs are 300nm-thick aligned sections with a 2.549 pixel size that cover complete cells. Subpositions were acquired at 0.78 nm pixel size.The serial tomograms were reconstructed, aligned and segmented using the IMOD package. IMOD was also used for manual segmentations.

Aligned EM tomographs covering an embryonic tracheal terminal cell, overview image /data/EMPIAR/C01/join tif micrographs - multiframe TIFF TIFF 1 1557 UNSIGNED BYTE variable 25.49 variable 25.49

Tilt series were performed in 1 degree increments from 60 to -60 degrees with 2.549 nm pixel size on a FEI Tecnai F30 electron microscope with a Gatan OneView camera. The serial tomograms were reconstructed and aligned using the IMOD package.

Aligned EM tomographs covering an embryonic tracheal terminal cell, overview image /data/EMPIAR/C02/join tif micrographs - multiframe TIFF TIFF 1 2284 UNSIGNED BYTE variable 25.49 variable 25.49

Tilt series were performed in 1 degree increments from 60 to -60 degrees with 2.549 nm pixel size on a FEI Tecnai F30 electron microscope with a Gatan OneView camera. The serial tomograms were reconstructed and aligned using the IMOD package.

Aligned EM tomographs covering an embryonic tracheal terminal cell after 1 hour of dynamin inactivation /data/EMPIAR/DynaminInactivation-1hr/join tif micrographs - multiframe TIFF TIFF 1 1166 UNSIGNED BYTE variable 25.49 variable 25.49

Tilt series were performed in 1 degree increments from 60 to -60 degrees with 2.549 nm pixel size on a FEI Tecnai F30 electron microscope with a Gatan OneView camera. The serial tomograms were reconstructed and aligned using the IMOD package.

Aligned EM tomographs covering an embryonic tracheal terminal cell after 1 hour of dynamin inactivation /data/EMPIAR/DynaminInactivation-2hrs/tiff join micrographs - multiframe TIFF TIFF 1 438 UNSIGNED BYTE variable 25.49 variable 25.49

Tilt series were performed in 1 degree increments from 60 to -60 degrees with 2.549 nm pixel size on a FEI Tecnai F30 electron microscope with a Gatan OneView camera. The serial tomograms were reconstructed and aligned using the IMOD package.

Tomographs covering subpositions within an embryonic tracheal terminal cell /data/EMPIAR/C01/subpositions micrographs - multiframe MRC MRC 45 200 UNSIGNED BYTE 4096 7.8 4096 7.8

Data is organized by grids (g*) where the physical sections (s*) were present; hm* refers to the number of high-magnification images obtained from a given section. Tomograms are .mrc files, saved as .rec files. 3D reconstruction models are saved in the same folder as .mod and are also .mrc files generated using IMOD.

data/EMPIAR/C01/subpositions/left_g4*/g4*s*hm*/left_g4*s*_hm*.rec data/EMPIAR/C01/subpositions/left_g4*/g4*s*hm*/left_g4*s*_hm*.mod data/EMPIAR/C01/subpositions/left_g4*/g4*s*hm* Tomographs covering subpositions within an embryonic tracheal terminal cell /data/EMPIAR/C02/subpositions micrographs - multiframe MRC MRC 36 200 UNSIGNED BYTE 4096 7.8 4096 7.8

Data is organized by grids (g*) where the physical sections (s*) were present; hm* refers to the number of high-magnification images obtained from a given section. Tomograms are .mrc files, saved as .rec files. 3D reconstruction models are saved in the same folder as .mod and are also .mrc files generated using IMOD.

data/EMPIAR/C02/subpositions/g4*/g4*s*hm*/g4*s*hm*.rec data/EMPIAR/C02/subpositions/g4*/g4*s*hm*/g4*s*hm*.mod data/EMPIAR/C02/subpositions/g4*/g4*s*hm*