Single-particle cryo-EM of the human CDK-activating kinase in complex with ATP-gamma-S

REL 2020-06-08 2020-10-02 2020-10-02 Single-particle cryo-EM of the human CDK-activating kinase in complex with ATP-gamma-S 0000-0001-9379-7159 Basil J Greber QB3, University of California Berkeley California United States 94720 0000-0001-9816-3681 Eva Nogales Universtity of California, Berkeley and Howard Hughes Medical Institute Berkeley United States 94720 Greber BJ Perez-Bertoldi JM Lim K Iavarone AT Toso DB Nogales E 3.5 10.6019/EMPIAR-10431 EMDB EMD-22123 Greber BJ Perez-Bertoldi JM Lim K Iavarone AT Toso DB Nogales E The cryoelectron microscopy structure of the human CDK-activating kinase Proceedings of the National Academy of Sciences of the United States of America Proc. Natl. Acad. Sci. U.S.A. United States 2020 English 10.1073/pnas.2009627117 32855301

The micrographs are unaligned 69-frame TIFF-format movies with a total electron exposure of 69 electrons per Angstrom squared. Movies that are empty (i.e. broken holes) or do not contain any useful data for other reasons have been removed from the dataset.

Unaligned movies of human CAK, dataset 1 /data/movies/20feb11_dataset1 micrographs - multiframe TIFF TIFF 2967 69 UNSIGNED BYTE 11520 0.343 8184 0.343

Unaligned super-resolution electron micrograph movies. 69 frames per movie with an accumulated exposure of 69 electrons per Angstrom squared. Movies with numbers up to 178 are already gain corrected. All other movies are not gain corrected (gain reference for these is provided).

Unaligned movies of human CAK, dataset 2 /data/movies/20mar13_dataset2 micrographs - multiframe TIFF TIFF 3273 69 UNSIGNED BYTE 11520 0.343 8184 0.343

Unaligned super-resolution electron micrograph movies without gain correction (gain reference is provided). 69 frames per movie with an accumulated exposure of 69 electrons per Angstrom squared.