REL 2020-05-06 2020-05-13 2023-01-23 Patrick Cramer Max-Planck-Institute for Biophysical Chemistry Göttingen Germany 37077 Patrick Cramer 0000-0001-5454-7755 Göttingen Germany 37077 Hillen HS Kokic G Farnung L Dienemann C Tegunov D Cramer P 3.0 10.6019/EMPIAR-10409 EMDB EMD-11007 Hillen HS Kokic G Farnung L Dienemann C Tegunov D Cramer P Nature Nature 2020 English 10.1038/s41586-020-2368-8 32438371 Unaligned TIF movies of SARS-CoV2 RdRp in complex with nsp7, nsp8 and RNA (part 1) /data/data_tilt30_round1 micrographs - multiframe TIFF TIFF 3092 80 UNSIGNED BYTE 5760 0.834 4092 0.834

Movies are from K3 (non-superres) and uncorrected (gain can be found in ./gain subfolders). Each LZW compressed.tif movie is accompanied with its respective .xml metadata file generated by Warp. Movies contain 80 frames collected over 2.2 seconds with a total dose of 60 e-/A2. Data was collected at 30 degree stage tilt. Particle locations were picked with Warp (locations are stored in ./matching subfolder). Micrograph thumbnails can be found in ./thumbnails subfolders.

data/data_tilt30_round1/HH691_funky_RNA_tilt30_*.tif data/data_tilt30_round1/matching/HH691_funky_RNA_tilt30_*_SARSCoV2_nsp12_net_4.star data/data_tilt30_round1/matching/ Unaligned TIF movies of SARS-CoV2 RdRp in complex with nsp7, nsp8 and RNA (part 2) /data/data_tilt30_round2 micrographs - multiframe TIFF TIFF 5076 80 UNSIGNED BYTE 5760 0.834 4092 0.834

Movies are from K3 (non-superres) and uncorrected (gain can be found in ./gain subfolders). Each LZW compressed.tif movie is accompanied with its respective .xml metadata file generated by Warp. Movies contain 80 frames collected over 2.2 seconds with a total dose of 60 e-/A2. Data was collected at 30 degree stage tilt. Particle locations were picked with Warp (locations are stored in ./matching subfolder). Micrograph thumbnails can be found in ./thumbnails subfolders.

data/data_tilt30_round2/HH691_grid20_tilt30_*.tif data/data_tilt30_round2/matching/HH691_grid20_tilt30_*_SARSCoV2_nsp12_net_4.star data/data_tilt30_round2/matching Warp picked and extracted particles /data/particles picked particles - single frame - processed MRCS MRCS 1385860 1 32 BIT FLOAT 240 0.834 240 0.834

Particles were picked and extracted using Warp.

data/all_SARSCoV2_nsp12_net_4.star data/particles Reextraced particles at 1.3 A/px (for Map2 and Map3) /data/particles picked particles - single frame - processed MRCS MRCS 415690 1 32 BIT FLOAT 256 1.3 256 1.3

Particles from Map1 were reextraced using Relion 3 at 1.3 A/px. Refer to ED Figure 1 of the paper.

data/all_reextract160A.star data/particles Refined particles (Map 1) /data/particles picked particles - single frame - processed MRCS MRCS 417736 1 32 BIT FLOAT 240 0.834 240 0.834

Refined particles for Map1 (refer to ED Figure 1 of the paper).

data/Map1_Refine3D.star data/particles Refined particles (Map 2) /data/particles picked particles - single frame - processed MRCS MRCS 172369 1 32 BIT FLOAT 256 1.3 256 1.3

Refined particles for Map2 (refer to ED Figure 1 of the paper).

data/Map2_Refine3D.star data/particles Refined particles (Map 3) /data/particles picked particles - single frame - processed MRCS MRCS 172369 1 32 BIT FLOAT 256 1.3 256 1.3

Refined particles for Map3 (refer to ED Figure 1 of the paper).

data/Map3_Refine3D.star data/particles