REL
2020-04-06
2020-07-06
2020-08-25
Subnanometer-resolution structure determination in situ by a hybrid subtomogram averaging - single particle cryoEM - workflow - on TMV
0000-0003-3550-6274
Mikhail
Kudryashev
Max-Planck-Institut für Biophysik
Max von Laue Straße 3
Frankfurt am Main
Germany
60438
0000-0003-3550-6274
Mikhail
Kudryashev
Max-Planck-Institut für Biophysik
Max von Laue Straße 3
Frankfurt am Main
Germany
60438
Sanchez RM
Zhang Y
Chen W
Dietrich L
Kudryashev M
37.5
10.6019/EMPIAR-10393
EMDB
EMD-10834
Sanchez RM
Zhang Y
Chen W
Dietrich L
Kudryashev M
Subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM
Nature communications
Nat Commun
Germany
1
11
2020
English
10.1038/s41467-020-17466-0
32709843
A developed workflow for subtomogram averaging (StA) from cryo electron tomograms (cryo-ET), with combination and beneficial from cryo single particle analysis (cryo-SPA).
TMV-hybrid tomography- tiltstacks- aligned and CTF corrected
/data/tmv-hybrid-tiltstacks
tilt series
MRC
MRC
4
41
32 BIT FLOAT
7420
1.1
7676
1.1
TMV testing data - hybrid tiltstacks - motioncor2 aligned and CTF corrected
Subtomogram averaging table - sta_rslt.tbl
Script for creating refine particle stack from StA table - create_stack_b1.m
Refine particle stack - hyb_b1.mrcs
Refine particle star file - hyb_b1.star
Refine helical parameter - refine_command_b1.txt