REL 2020-05-13 2020-05-14 2021-02-12 Three-dimensional ultrastructure of Plasmodium falciparum throughout cytokinesis 0000-0002-5883-7271 Jeffrey Dvorin Division of Infectious Diseases, Boston Children's Hospital and Department of Pediatrics, Harvard Medical School 300 Longwood Avenue Boston MA United States 02115 0000-0002-5883-7271 Jeffrey Dvorin Division of Infectious Diseases, Boston Children's Hospital and Department of Pediatrics, Harvard Medical School 300 Longwood Avenue Boston MA United States 02115 Rudlaff RM Kraemer S Marshman J Dvorin JD 3.9 10.6019/EMPIAR-10392 FIB-SEM Rudlaff RM Kraemer S Marshman J Dvorin JD Three-dimensional ultrastructure of Plasmodium falciparum throughout cytokinesis PLoS pathogens PLoS Pathog 6 16 2020 English 10.1371/journal.ppat.1008587 32511279
Each file is FIB-SEM data for a single parasite. Data were aligned via cross-correlation, denoised with the non-local means filter, and binned using an in-house Matlab-based software package yielding volumes with a voxel size of 10 x 10 x 20 nm3.
 S1_File_Early_Segmentation_1 /data micrographs - multiframe MRC MRC 311 1 32 BIT FLOAT 530 100.0 810 100.0
File S1: Volume EM of Plasmodium falciparum 3D7 early schizont 1. Aligned, denoised, binned, and cropped serial slices of an early schizont from the [+]E64-treated 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).
 S2_File_Early_Segmentation_2 /data micrographs - multiframe MRC MRC 397 1 UNSIGNED 16 BIT INTEGER 773 100.0 607 100.0
File S2: Volume EM of Plasmodium falciparum 3D7 early schizont 2. Aligned, denoised, binned, and cropped serial slices of an early schizont from the [+]E64-treated 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).
 S3_File_Early_Segmentation_A /data micrographs - multiframe MRC MRC 427 1 UNSIGNED 16 BIT INTEGER 611 100.0 805 100.0
File S3: Volume EM of Plasmodium falciparum 3D7 early schizont A. Aligned, denoised, binned, and cropped serial slices of an early schizont from the [-]E64 46-48hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).
 S4_File_Early_Segmentation_B /data micrographs - multiframe MRC MRC 382 1 UNSIGNED 16 BIT INTEGER 621 100.0 798 100.0
File S4: Volume EM of Plasmodium falciparum 3D7 early schizont B. Aligned, denoised, binned, and cropped serial slices of an early schizont from the [-]E64 46-48hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).
 S5_File_Mid_Segmentation_1 /data micrographs - multiframe MRC MRC 278 1 32 BIT FLOAT 655 100.0 718 100.0
File S5: Volume EM of Plasmodium falciparum 3D7 mid-segmentation schizont 1. Aligned, denoised, binned, and cropped serial slices of a mid-segmentation schizont from the [+]E64-treated 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).
 S6_File_Mid_Segmentation_2 /data micrographs - multiframe MRC MRC 278 1 UNSIGNED 16 BIT INTEGER 657 100.0 726 100.0
File S6: Volume EM of Plasmodium falciparum 3D7 mid-segmentation schizont 2. Aligned, denoised, binned, and cropped serial slices of a mid-segmentation schizont from the [+]E64-treated 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).
 S7_File_Mid_Segmentation_A /data micrographs - multiframe MRC MRC 249 1 UNSIGNED 16 BIT INTEGER 838 100.0 701 100.0
File S7: Volume EM of Plasmodium falciparum 3D7 mid-segmentation schizont A. Aligned, denoised, binned, and cropped serial slices of a mid-segmentation schizont from the [-]E64 46-48hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).
 S8_File_Mid_Segmentation_B /data micrographs - multiframe MRC MRC 290 1 UNSIGNED 16 BIT INTEGER 830 100.0 651 100.0
File S8: Volume EM of Plasmodium falciparum 3D7 mid-segmentation schizont B. Aligned, denoised, binned, and cropped serial slices of a mid-segmentation schizont from the [-]E64 46-48hpi block. Voxels 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).
 S9_File_PVM_Rupture /data micrographs - multiframe MRC MRC 190 1 32 BIT FLOAT 617 100.0 617 100.0
File S9: Volume EM of Plasmodium falciparum 3D7 schizont at PVM rupture. Aligned, denoised, binned, and cropped serial slices of a schizont at the PVM rupture stage from the [+]E64 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).
 S10_File_Post_PVM_Rupture_E64 /data micrographs - multiframe MRC MRC 306 1 32 BIT FLOAT 684 100.0 649 100.0
File S10: Volume EM of Plasmodium falciparum 3D7 post-PVM rupture schizont. Aligned, denoised, binned, and cropped serial slices of a post-PVM rupture schizont from the [+]E64-treated block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. cellular debris).
 S11_File_Post_PVM_Rupture_NoE64 /data micrographs - multiframe TIFF TIFF 339 1 UNSIGNED BYTE 1005 100.0 870 100.0
File S11: Volume EM of Plasmodium falciparum 3D7 post-PVM rupture schizont. Aligned, denoised, binned, and cropped serial slices of a post-PVM rupture schizont from the [-]E64-treated block. Voxels are 10nm x 10nm x 20nm. The image quality of this cell is relatively lower; thus, it was not rendered.