REL
2020-04-12
2020-05-29
2020-05-29
Single-Particle Cryo-EM of Arabinofuranosyltransferase AftD from Mycobacteria, Mutant R1389S
0000-0001-6656-6320
Yong Zi
Tan
Department of Physiology and Cellular Biophysics, Columbia University
1150 Saint Nicolas Ave
New York
New York
United States
10032
0000-0003-3293-2200
Filippo
Mancia
Department of Physiology and Cellular Biophysics, Columbia University
1150 Saint Nicolas Ave
New York
New York
United States
10032
0000-0002-0624-5020
Bridget
Carragher
New York Structural Biology Center/Columbia University
89 Convent Ave
New York
New York
United States
10027
0000-0003-2394-0831
Clinton
S
Potter
New York Structural Biology Center/Columbia University
89 Convent Ave
New York
New York
United States
10027
Tan YZ
Zhang L
Rodrigues J
Zheng RB
Giacometti SI
Rosário AL
Kloss B
Dandey VP
Wei H
Brunton R
Raczkowski AM
Athayde D
Catalão MJ
Pimentel M
Clarke OB
Lowary TL
Archer M
Niederweis M
Potter CS
Carragher B
Mancia F
2.3
10.6019/EMPIAR-10391
EMDB
EMD-21600
EMD-21601
Tan YZ
Zhang L
Rodrigues J
Zheng RB
Giacometti SI
Rosário AL
Kloss B
Dandey VP
Wei H
Brunton R
Raczkowski AM
Athayde D
Catalão MJ
Pimentel M
Clarke OB
Lowary TL
Archer M
Niederweis M
Potter CS
Carragher B
Mancia F
Cryo-EM Structures and Regulation of Arabinofuranosyltransferase AftD from Mycobacteria
Molecular cell
Mol. Cell
2020
English
10.1016/j.molcel.2020.04.014
32386575
Unaligned and compressed multi-frame movies
/data/01_Raw_Frames
micrographs - multiframe
TIFF
TIFF
4889
90
1
90
UNSIGNED 16 BIT INTEGER
3838
1.0605
3710
1.0605
Data collection was performed using a dose of ~96.78 e-/Å2 across 90 frames (150 msec per frame) at a dose rate of ~8.34 e-/pix/sec, using a set defocus range of -0.8 μm to -1.6 μm. Raw frames were compressed using pbzip2. Gain reference image is 01_Raw_Frames/18nov03b/rawdata/references/18nov02f_03102315_01_3838x3710_norm_0_mod.mrc.
data/02_Aligned_Micrographs/*mrc
data/03_Final_Stack/AftD-R1389S_All-Particles.star
data/03_Final_Stack
Aligned and dose-weighted micrographs
/data/02_Aligned_Micrographs
micrographs - single frame
MRC
MRC
4888
1
32 BIT FLOAT
3838
1.0605
3710
1.0605
Movie frames were aligned using MotionCor2 with 5 by 5 patches and B-factor of 100 through the Appion software package.
data/02_Aligned_Micrographs/*mrc
data/03_Final_Stack/AftD-R1389S_All-Particles.star
data/03_Final_Stack
Final Particle Stacks with Refined Euler Angles and Shifts (Overall and Focused Refined on CBM3)
/data/03_Final_Stack
picked particles - single frame - processed
MRCS
MRCS
3
1
32 BIT FLOAT
256
1.0605
256
1.0605
Final particle stacks, with Relion .star file containing the refined Euler angles and shifts. AftD-R1389S_All-Particles comprise of all particles while AftD-R1389S_Class1 comprise of particles going into Class 1 and AftD-R1389S_Class2 comprise of particles going into Class 2.
data/02_Aligned_Micrographs/*mrc
data/03_Final_Stack/AftD-R1389S_All-Particles.star
data/03_Final_Stack