REL
2020-03-09
2020-04-09
2022-01-31
Cryo-EM structure of Lumazine Synthase
0000-0003-2096-8310
David
Bhella
MRC-University of Glasgow Centre for Virus Research
464 Bearsden Road
Glasgow
Glasgow
United Kingdom
G61 1QH
0000-0003-2096-8310
David
Bhella
MRC-University of Glasgow Centre for Virus Research
464 Bearsden Road
Glasgow
Glasgow
United Kingdom
G61 1QH
Bhella D
Streetley J
Clarke M
Cowton V
Patel A
3.0
10.6019/EMPIAR-10384
EMDB
EMD-10736
Bhella D
Cryo-electron microscopy: an introduction to the technique, and considerations when working to establish a national facility
Biophysical reviews
Biophys Rev
4
11
515
519
2019
English
10.1007/s12551-019-00571-w
31359340
Frame aligned multi-frame movies of Lumazine Synthase imaged on a CryoARM300 and DE64
/data
micrographs - multiframe
MRC
MRC
1198
41
1
41
32 BIT FLOAT
4096
0.597
4096
0.597
Lumazine Synthase was expressed in E.coli and purified by immobilised metal affinity chromatography. Particles were frozen on C-flat R1.2/1.3 grids. Imaging was performed at 200 kX magnification on a JEOL CryoARM 300 equipped with a Direct Electron DE64 detector. Energy filtered images were collected with a slit-width of 20eV using JADAS. A single image was recorded per hole using electron counting with two times binning.