REL
2020-02-06
2020-04-14
2020-04-14
Cryo-electron tomography of E. coli minicells
0000-0002-9341-2295
Alister
Burt
Institut de Biologie Structurale
Grenoble
France
38000
0000-0002-1908-3921
Irina
Gutsche
Institut de Biologie Structurale
Grenoble
France
38000
Burt A.
Desfosses A.
Gutsche I.
Clare D. K
141.7
10.6019/EMPIAR-10364
EMDB
EMD-10160
Burt A
Cassidy CK
Ames P
Bacia-Verloop M
Baulard M
Huard K
Luthey-Schulten Z
Desfosses A
Stansfeld PJ
Margolin W
Parkinson JS
Gutsche I
Complete structure of the chemosensory array core signalling unit in an E. coli minicell strain
Nature communications
Nat Commun
1
11
2020
English
10.1038/s41467-020-14350-9
32029744
Motile bacteria sense chemical gradients with transmembrane receptors organised in supramolecular signalling arrays. Understanding stimulus detection and transmission at the molecular level requires precise structural characterisation of the array building block known as a core signalling unit. Here we introduce an Escherichia coli strain that forms small minicells possessing extended and highly ordered chemosensory arrays. We use cryo-electron tomography and subtomogram averaging to provide a three-dimensional map of a complete core signalling unit, with visible densities corresponding to the HAMP and periplasmic domains. This map, combined with previously determined high resolution structures and molecular dynamics simulations, yields a molecular model of the transmembrane core signalling unit and enables spatial localisation of its individual domains. Our work thus offers a solid structural basis for the interpretation of a wide range of existing data and the design of further experiments to elucidate signalling mechanisms within the core signalling unit and larger array.
Unaligned multi-frame micrographs from tilt series containing WM4196 minicells
/data
micrographs - multiframe
MRC
MRC
17
5
SIGNED 16 BIT INTEGER
3838
2.243
3710
2.243
Information on each tilt series in corresponding mdoc files in the mdoc directory.
Data was collected by Daniel Clare on a Titan Krios at eBic, Diamond Light Source, UK.
Each image is taken at ca. 0.2um defocus with a volta phase plate.
Dose per tilt image 1.0 e/A2
Each tilt image is gain corrected
8x binned tomograms with the SIRT-like filter are also provided
This data was used for subtomogram averaging of the in situ chemotaxis receptor array - https://doi.org/10.1038/s41467-020-14350-9