REL 2020-02-12 2020-05-27 2020-05-27 0000-0002-4321-6253 Dominika Borek UT Southwestern Medical Center 5323 Harry Hines Blvd. Dallas TX United States 75390 0000-0002-4321-6253 Dominika Borek UT Southwestern Medical Center 5323 Harry Hines Blvd. Dallas TX United States 75390 0000-0003-3640-8545 Zbyszek Otwinowski UT Southwestern Medical Center 5323 Harry Hines Blvd. Dallas TX United States 75390 Bromberg R Guo Y Borek D Otwinowski Z 1.3 10.6019/EMPIAR-10362 EMDB EMD-21376 Bromberg R Guo Y Borek D Otwinowski Z IUCrJ IUCrJ Pt 3 7 445 452 2020 English 10.1107/s2052252520002444

1. Talos Arctica 200 kV, K2 Gatan, detector pixel size 0.91 Angstrom (nominal mag 45k), data pixel size 0.455, no phase plate, no objective aperture (due to instability of its position during data collection), 2. large defocus (-2.8 to -1.2 um) , large coma (7.0 mrad), trefoil (0.79 um), anisotropic magnification (~3%) 3. 257 unaligned movies, each contains 100 raw movie frames with frames exposed 0.4 s/frame with dose ~0.9 electron per squared Angstrom per frame 5. Particle symmetry - C5, mass 144 kDa, very strong preferred orientation 6. Final reconstruction without polishing 2.6 Angstrom (0.143 FSC criterion)

Unaligned multiframe data for 2.6 Angstrom cryo-EM reconstructions of HemQ from Geobacillus in the presence of substantial aberrations /data/T0T_grid6 micrographs - multiframe MRCS MRCS 257 100 1 100 UNSIGNED BYTE 7676 0.455 7420 0.455

Image height and width may have to be swapped.