REL
2020-02-10
2020-05-27
2020-05-27
2.7 Angstrom cryo-EM reconstructions of glucose isomerase in the presence of substantial aberrations
0000-0002-4321-6253
Dominika
Borek
UT Southwestern Medical Center
5323 Harry Hines Blvd.
Dallas
TX
United States
75390
0000-0003-3640-8545
Zbyszek
Otwinowski
UT Southwestern Medical Center
5323 Harry Hines Blvd.
Dallas
TX
United States
75390
0000-0002-4321-6253
Dominika
Borek
UT Southwestern Medical Center
5323 Harry Hines Blvd.
Dallas
TX
United States
75390
Bromberg R
Guo Y
Borek D
Otwinowski Z
1.0
10.6019/EMPIAR-10360
EMDB
EMD-21371
Bromberg R
Guo Y
Borek D
Otwinowski Z
High-resolution cryo-EM reconstructions in the presence of substantial aberrations
IUCrJ
IUCrJ
Pt 3
7
445
452
2020
English
10.1107/s2052252520002444
1. Talos Arctica 200 kV, K2 Gatan, detector pixel size 0.91 Angstrom (nominal mag 45k), no phase plate, no objective aperture (due to instability of its position during data collection),
2. large defocus () , large coma (5.2 mrad), minimal trefoil (0.49 um), anisotropic magnification ()
3. 202 unaligned movies, each contains 200 raw movie frames with frames exposed 0.5 s/frame with dose ~0.6 electron per squared Angstrom per frame
5. Particle symmetry - D2, mass 172 kDa
6. Final reconstruction without polishing 2.7 Angstrom (0.143 FSC criterion)
Unaligned multiframe data for xylose isomerase (glucose isomerase)
/data
micrographs - multiframe
MRCS
MRCS
202
200
1
200
UNSIGNED 16 BIT INTEGER
3838
0.91
3710
0.91
Image width and height may need to be swapped.