REL
2020-02-03
2020-11-23
2020-11-23
Cryo-EM of PAC1 receptor bound to PACAP38 and Gs protein
Denise
Wootten
Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University
399 Royal Parade
Parkville
Victoria
Australia
3052
Denise
Wootten
Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University
399 Royal Parade
Parkville
Victoria
Australia
3052
Liang YL
Belousoff MJ
Zhao P
Koole C
Fletcher MM
Truong TT
Julita V
Christopoulos G
Xu HE
Zhang Y
Khoshouei M
Christopoulos A
Danev R
Sexton PM
Wootten D
4.8
10.6019/EMPIAR-10359
EMDB
EMD-0993
EMD-20278
Liang YL
Belousoff MJ
Zhao P
Koole C
Fletcher MM
Truong TT
Julita V
Christopoulos G
Xu HE
Zhang Y
Khoshouei M
Christopoulos A
Danev R
Sexton PM
Wootten D
Toward a Structural Understanding of Class B GPCR Peptide Binding and Activation
Molecular cell
Mol. Cell
Australia
3
77
656
668.e5
2020
English
10.1016/j.molcel.2020.01.012
32004469
The movies were collected in counting mode with gain normalization and saved as compressed TIFFs by SerialEM.
Unaligned multi-frame gain-normalized movies in LZW compressed TIFF format
/data/PS_20_2_4
micrographs - multiframe
TIFF
TIFF
7649
62
1
62
UNSIGNED BYTE
5760
0.83
4092
0.83
- Approximately half of the data were collected with the Volta phase plate (VPP). Such movies are in the "VPP" subfolder and contain "VPP" in their filename.
- The movies were collected with a 9-hole beam-image shift acquisition pattern. The filename contains a four digit number in the range 0000 to 0008 corresponding to the image-shift acquisition position.
- The image-shift induced beam-tilt was not compensated optically during acquisition. For best results, perform per-image-shift-position beam-tilt measurement and correction with CTF refinement.
- The movies were recorded with Gatan K3 camera in counting (hardware bin by 2) mode and saved as gain-normalized LZW compressed TIFF files by SerialEM.