REL 2020-01-21 2020-03-20 2020-01-23 0000-0003-3550-6274 Misha Kudryashev Max Planck Institute for Biophysics Max von Laue Strasse 3 Frankfurt am Main Germany 60438 0000-0003-3550-6274 Misha Kudryashev Max Planck Institute for Biophysics Max von Laue Strasse 3 Frankfurt am Main Germany 60438 Chen W Kudryashev M 254.2 10.6019/EMPIAR-10349 EMDB EMD-10637 EMD-10638 EMD-10639 EMD-10640 EMD-10641 EMD-10642 EMD-10643 EMD-10644 EMD-10645 EMD-10646 Chen W Kudryashev M EMBO reports EMBO Rep. Germany 2020 English 10.15252/embr.201949891 32147968 82 tilt series (frames aligned my motioncorr2) in .st stacks (mrc format for imod) including .tlt, .xf and .defocus files /data/deposition_empiar tilt series MRC MRC 82 1 32 BIT FLOAT 3710 2.7 3838 2.7

These are tilt series which we used for generation of the 12.6 A structure or RyR1 in native membranes. the notable repeating molecules also include ATP synthase and a transporter SERCA. Details about the RYR1 structure can be found in the publication. Some tomograms have a higher exposure for the untilted micrograph which we use for "hybrid StA". The particle folder contains 2547 unbinned CTF-corrected volumes which were used to generate the structure. The particles have not been exposure filtered. There is a Dynamo-style *.tbl which has metadata for the particles and the alignment parameters. Column 34 has class assignments for classification into 4 classes.

data/deposition_empiar/*st data/deposition_particles/*tbl data/deposition_particles