REL
2020-01-09
2020-03-10
2020-03-10
Cryo-EM of GLP-1 receptor bound to TT-OAD2 non-peptidic agonist
Denise
Wootten
Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University
399 Royal Parade
Parkville
Victoria
Australia
3052
Denise
Wootten
Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University
399 Royal Parade
Parkville
Victoria
Australia
3052
Zhao P
Liang YL
Belousoff MJ
Deganutti G
Fletcher MM
Willard FS
Bell MG
Christe ME
Sloop KW
Inoue A
Truong TT
Clydesdale L
Furness SGB
Christopoulos A
Wang MW
Miller LJ
Reynolds CA
Danev R
Sexton PM
Wootten D
6.2
10.6019/EMPIAR-10346
EMDB
EMD-20179
Zhao P
Liang YL
Belousoff MJ
Deganutti G
Fletcher MM
Willard FS
Bell MG
Christe ME
Sloop KW
Inoue A
Truong TT
Clydesdale L
Furness SGB
Christopoulos A
Wang MW
Miller LJ
Reynolds CA
Danev R
Sexton PM
Wootten D
Activation of the GLP-1 receptor by a non-peptidic agonist
Nature
Nature
7790
577
432
436
2020
English
10.1038/s41586-019-1902-z
31915381
Original gain-normalized moves in LZW-compressed TIFF format.
Unaligned multi-frame movies of GLP-1R-TT-OAD2 complex
/data/20181213_PS2.3.3
micrographs - multiframe
TIFF
TIFF
4259
67
UNSIGNED BYTE
11520
0.413
8184
0.413
- Approximately two-thirds of the data were collected with the Volta phase plate (VPP). "VPP" in the filename indicates that the movie was collected with the phase plate.
- Most movies were collected with a 9-hole beam-image shift acquisition pattern. The filename of such movies contains a four digit number in the range 0000 to 0008 corresponding to the image-shift acquisition position.
- The image-shift induced beam-tilt was not compensated optically during acquisition. For best results, perform per-image-shift position beam-tilt measurement and correction with CTF refinement.
- The movies were recorded with Gatan K3 camera in super-resolution mode and saved as gain-normalized LZW compressed TIFF files by SerialEM.
- Accelerating voltage: 300 kV
- Spherical aberration: 2.7 mm
- SuperRes pixel size: 0.413 A/superpixel
- Physical pixel size: 0.826 A/physpix
- Exposure rate: 10.9 e/physpix/s
- Exposure rate: 16.0 e/A^2/s
- Exposure time: 4.015 s
- Total exposure: 64.2 e/A^2
- Number of frames: 67
- Defocus range: 0.3 - 1.4 um
Unaligned multi-frame movies of GLP-1R-TT-OAD2 complex
/data/PS.TT.3
micrographs - multiframe
TIFF
TIFF
3159
62
UNSIGNED BYTE
5760
0.826
4092
0.826
- The movies were recorded with Gatan K3 camera in counting (non-superres) mode and saved as gain-normalized LZW compressed TIFF files by SerialEM.
- All movies were collected with a 9-hole beam-image shift acquisition pattern. The filename contains a four digit number in the range 0000 to 0008 corresponding to the image-shift acquisition position.
- The image-shift induced beam-tilt was not compensated optically during acquisition. For best results, perform per-image-shift position beam-tilt measurement and correction with CTF refinement.
- Accelerating voltage: 300 kV
- Spherical aberration: 2.7 mm
- Pixel size: 0.826 A/pix
- Exposure rate: 12.2 e/physpix/s
- Exposure rate: 17.9 e/A^2/s
- Exposure time: 3.715 s
- Total exposure: 66.5 e/A^2
- Number of frames: 62
- Defocus range: 0.6 - 1.5 um