CryoEM dataset containing multiple conformations of the asymmetric αVβ8 integrin bound to two Fabs on a holey carbon grid (minimal preferred orientations)

REL 2019-12-13 2020-01-31 2020-02-07 CryoEM dataset containing multiple conformations of the asymmetric αVβ8 integrin bound to two Fabs on a holey carbon grid (minimal preferred orientations) 0000-0003-1909-5751 Melody Grace Campbell UCSF 600 16th Street, Room S474 San Francisco CA United States 94143 0000-0001-9535-0369 Yifan Cheng Department of Biochemistry and Biophysics, University of California, San Francisco 600 16th Street, Room S472D San Francisco CA United States 94143 Campbell MG Cheng Y 882.1 10.6019/EMPIAR-10345 EMDB EMD-20795 Campbell MG Cormier A Ito S Seed RI Bondesson AJ Lou J Marks JD Baron J Cheng Y Nishimura SL Cryo-EM Reveals Integrin-Mediated TGF-β Activation without Release from Latent TGF-β Cell Cell 2020 English 10.1016/j.cell.2019.12.030 31955848 Unaligned 60-frame movies of αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid /data/Movies_18jam15a micrographs - multiframe TIFF TIFF 1644 60 UNSIGNED BYTE 7676 0.6725 7420 0.6725

αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid (particles have minimal preferred orientations): 1644 unaligned uncorrected movies were recorded automatically using SerialEM on a K2 camera in super-resolution (dstep=2.5) mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. The 12 second movies contain 60 frames and the super resolution pixel size is 0.6725 Å/px. The total dose per movie is ~55 e/Å^2. The gain reference anda file containing coordinates for a single dead column.

data/Movies_18jam15a/18ja****_****.tif data/Stack_18jam15a/Micrographs/18ja*_ali_DW_extract.star data/Stack_18jam15a/Micrographs Dose-weighted aligned micrographs of αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid /data/Micrographs_18jam15a micrographs - single frame MRC MRC 1644 1 32 BIT FLOAT 3838 1.345 3710 1.345

αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid (particles have minimal preferred orientations): 1644 aligned and dose-weighted (in MotionCor2) micrographs recorded automatically using SerialEM on a K2 camera (dstep=5) in super-resolution mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.345. The total dose per micrograph is ~55 e/Å^2.

data/Micrographs_18jam15a/18ja*_ali_DW.mrc data/Stack_18jam15a/Micrographs/18ja*_ali_DW_extract.star data/Stack_18jam15a/Micrographs Dose-weighted aligned particle stacks of αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid /data/Stack_18jam15a picked particles - single frame - processed MRCS MRCS 1642 1 32 BIT FLOAT 300 1.345 300 1.345

αVβ8 integrin bound to a Fab (C6D4) on a holey carbon grid (particles have minimal preferred orientations): Stacks of 84,266 dose-weighted (in MotionCor2) particles contributing to the seven conformational snapshots described in the publication . Particles were selected after 2D classification. Images were recorded automatically using SerialEM on a K2 camera (dstep=5) in super-resolution mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.345.

data/Micrographs_18jam15a/18ja****_****_ali_DW.mrc data/Stack_18jam15a/Micrographs/18ja****_****_ali_DW_extract.star data/Stack_18jam15a/Micrographs