REL 2019-11-29 2020-07-28 2021-10-15 0000-0002-8040-3648 Yang Lee MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Cambridgeshire United Kingdom CB2 0QH 0000-0002-2008-9183 Christopher G Tate MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Cambridgeshire United Kingdom CB2 0QH Lee Y Tate CG 4.0 10.6019/EMPIAR-10342 EMDB EMD-10515 Lee Y Warne T Nehmé R Pandey S Dwivedi-Agnihotri H Chaturvedi M Edwards PC García-Nafría J Leslie AGW Shukla AK Tate CG Nature Nature United Kingdom 2020 English 10.1038/s41586-020-2419-1 32555462 Unaligned multi-frame micrographs /data/Movies/190830 micrographs - multiframe TIFF TIFF 9026 50 1 50 UNSIGNED 16 BIT INTEGER 3838 1.1 3710 1.1

data/Movies/190830 contains unaligned movies related to collection "LMB Krios2-1". The "_hn_"-portion of the filename, where n=1-9, denotes the hole position within the 3x3 image-shift acquisition matrix. Two exposures per hole are differentiated by the time-stamp portion of the filename (~20 seconds apart). Micrographs were collected with a nominal stage-tilt of 30deg. The files were written as gain-unnormalised integer TIFF files by serialEM. The real pixel size is 1.1 Å/pix. The dose rate is 1.02 e/Å^2/frame. Please use data/Movies/GainRef/190830_gainref.mrc as gain reference without any rotation or flipping. The associated K2 defects file is data/Movies/Defect/190830_defects.txt.

data/Movies/190830/*.tif data/Subtracted/subtracted_particles.star data/Subtracted/ Unaligned multi-frame micrographs /data/Movies/190907 micrographs - multiframe TIFF TIFF 6808 50 1 50 UNSIGNED 16 BIT INTEGER 3838 1.1 3710 1.1

data/Movies/190907 contains unaligned movies related to collection "LMB Krios2-2". The "_hn_"-portion of the filename, where n=1-9, denotes the hole position within the 3x3 image-shift acquisition matrix. Two exposures per hole are differentiated by the time-stamp portion of the filename (~20 seconds apart). Micrographs were collected with a nominal stage-tilt of 30deg. The files were written as gain-unnormalised integer TIFF files by serialEM. The real pixel size is 1.1 Å/pix. The dose rate is 0.98 e/Å^2/frame. Please use data/Movies/GainRef/190907_gainref.mrc as gain reference without any rotation or flipping. The associated K2 defects file is data/Movies/Defect/190907_defects.txt.

data/Movies/190907/*.tif data/Subtracted/subtracted_particles.star data/Subtracted/ Unaligned multi-frame micrographs /data/Movies/190911 micrographs - multiframe TIFF TIFF 2747 50 1 50 UNSIGNED 16 BIT INTEGER 3838 1.047 3710 1.047

data/Movies/190911 contains unaligned movies related to collection "DLS eBIC Krios1". Two exposures per hole are differentiated by the two fields following "_Data_" in the filename. Micrographs were collected with a nominal stage-tilt of 30deg. The files were written as gain-unnormalised integer MRC files by EPU and then converted to TIFF files by mrc2tif in the IMOD suite. During this process, the pixel size in the header was lost. The real pixel size is 1.047 Å/pix. The dose rate is 0.9 e/Å^2/frame. Please use data/Movies/GainRef/190911_gainref.mrc as gain reference without any rotation or flipping.

data/Movies/190911/*.tif data/Subtracted/subtracted_particles.star data/Subtracted/ Nanodisc subtracted particles /data/Subtracted picked particles - multiframe - processed MRCS MRCS 403991 1 32 BIT FLOAT 240 1.1 240 1.1

Nanodisc-subtracted particles used for final reconstruction. /data/Masks/for_refinement.mrc is the mask used for refinement. /data/Masks/initial_subtraction.mrc is the mask used for initial subtraction of nanodisc density.

data/Subtracted data/Subtracted/subtracted_particles.star data/Subtracted Nanodisc-reinstated particles /data/Reverted picked particles - multiframe - unprocessed MRCS MRCS 403991 1 32 BIT FLOAT 240 1.1 240 1.1

Refined subtracted particles reverted to original particle images; suitable for calculating or refining the complex with nanodisc-signal contribution.

data/Reverted data/Reverted/reverted_particles.star data/Reverted/Particles/190*/