REL
2019-10-21
2019-12-20
2021-11-08
Single Particle Cryo-EM Reconstructions of Human FACT in Complex with Partially Assembled Sub-nucleosomes
0000-0001-6656-6320
Yong Zi
Tan
New York Structural Biology Center/Columbia University
89 Convent Ave
New York
New York
United States
10027
0000-0001-5136-5331
Karolin
Luger
Department of Biochemistry, University of Colorado at Boulder
596 UCB
Boulder
Colorado
United States
80305
Liu YL
Zhou KZ
Zhang NZ
Wei HW
Tan YZT
Zhang ZZ
Carragher BC
Potter CSP
D'Arcy SD
Luger KL
2.2
10.6019/EMPIAR-10333
EMDB
EMD-20840
EMD-20841
Liu YL
Zhou KZ
Zhang NZ
Wei HW
Tan YZT
Zhang ZZ
Carragher BC
Potter CSP
D'Arcy SD
Luger KL
FACT caught in the act of manipulating the nucleosome
Nature
Nature
United Kingdom
2019
English
10.1038/s41586-019-1820-0
31775157
Unaligned multi-frame micrographs
/data/01_Raw_Frames
micrographs - multiframe
TIFF
TIFF
8319
50
1
50
UNSIGNED 16 BIT INTEGER
3838
1.0961
3710
1.0961
Unaligned movies saved in TIF format. The first data collection (19feb16b) was performed using a dose of ~66.37 e-/Å2 across 50 frames (200 msec per frame) at a dose rate of ~8.0 e–/pix/sec, using a set defocus range of -1.9 μm to -2.0 μm. The second data collection (19may31e) was performed using a dose of ~ 63.61 e-/Å2 across 50 frames (200 msec per frame) at a dose rate of ~7.6 e–/pix/sec, using a set defocus range of -1.5 μm to -2.5 μm. The gain reference images for each data collection are in the respective references folder.
Aligned and dose-weighted micrographs
/data/02_Aligned_Micrographs
micrographs - single frame
MRC
MRC
6934
1
32 BIT FLOAT
3838
1.0961
3710
1.0961
Aligned and dose-weighted micrographs. Motion correction was performed using Relion 3.
data/02_Aligned_Micrographs/MotionCorr/job0*/Movies/*mrc
data/03_Particle_Stacks/Class*.star
data/03_Particle_Stacks
Final Particle Stacks for Class 1 and 2 with Euler Angles and Shifts
/data/03_Particle_Stacks
picked particles - single frame - processed
MRCS
MRCS
2
1
32 BIT FLOAT
200
1.403008
200
1.403008
Final particle stacks for both class 1 and 2 maps with corresponding Relion .star file containing the refined Euler angles and shifts.
data/02_Aligned_Micrographs/MotionCorr/job0*/Movies/*mrc
data/03_Particle_Stacks/Class*.star
data/03_Particle_Stacks