REL
2019-08-26
2019-11-06
2019-11-19
Cryo-EM dataset, containing multiple conformations of a heterodimeric ABC exporter TmrAB (EQ-mutant), incubated with a mixture of ATP and ADP
0000-0003-1101-5366
Arne
Moeller
Max Planck Institute of Biophysics
Max-von-Laue-Straße 3
Frankfurt am Main
Hessen
Germany
60438
0000-0003-1101-5366
Arne
Moeller
Max Planck Institute of Biophysics
Max-von-Laue-Straße 3
Frankfurt am Main
Hessen
Germany
60438
Januliene D
Hofmann S
Mehdipour AR
Thomas C
Hummer G
Tampé R
Moeller A
7.1
10.6019/EMPIAR-10317
EMDB
EMD-4775
EMD-4776
Hofmann S
Januliene D
Mehdipour AR
Thomas C
Stefan E
Brüchert S
Kuhn BT
Geertsma ER
Hummer G
Tampé R
Moeller A
Conformation space of a heterodimeric ABC exporter under turnover conditions
Nature
Nature
7766
571
580
583
2019
English
10.1038/s41586-019-1391-0
31316210
Unaligned, gain-corrected, multi-frame movies together with picked particles, pre-cleaned in a single step of 3D-classification
Unaligned multi-frame micrographs of a heterodimeric ABC exporter TmrAB (EQ-mutant) in multiple ATP/ADP bound conformations
/data/Micrographs
micrographs - multiframe
MRC
MRC
3263
40
1
40
32 BIT FLOAT
3838
1.077
3710
1.077
Unaligned, gain-corrected, 40-frame movies, recorded automatically with EPU, using K2 camera in counting mode on a Titan Krios microscope operated at 300 kV at a nominal magnification of 130 000, corresponding to a pixel size of 1.077 Å. The total electron dose per micrograph - approximately 62 electrons per squared Angstrom.
data/Micrographs/*.mrc
data/Particles/1p5mln-TmrAB-stack.star
data/Particles
TmrAB(EQ) particles in multiple conformations after one round of cleaning in 3D
/data/Particles
picked particles - multiframe - unprocessed
MRCS
MRCS
1547017
1547017
32 BIT FLOAT
256
1.077
256
1.077
1.5 mln particle stack (box size - 256 pixels, 1.077 Å/pixel) of TmrAB(EQ) in multiple conformations after a single round of cleaning in 3D (particles picked and extracted in Relion-3).
data/Micrographs/*.mrc
data/Particles/1p5mln-TmrAB-stack.star
data/Particles