REL
2019-07-24
2019-10-14
2019-10-14
PolyA polymerase module of the cleavage and polyadenylation factor (CPF) from Saccharomyces cerevisiae
0000-0003-1815-3710
Passmore
LA
MRC laboratory of molecular biology
Francis Crick Avenue
Cambridge
Cambridgeshire
United Kingdom
CB2 0QH
0000-0003-1815-3710
Passmore
LA
MRC laboratory of molecular biology
Francis Crick Avenue
Cambridge
Cambridgeshire
United Kingdom
CB2 0QH
Casanal A
Kumar A
Hill CH
Emsley P
Passmore LA
15.4
10.6019/EMPIAR-10299
EMDB
EMD-3908
Casañal A
Kumar A
Hill CH
Easter AD
Emsley P
Degliesposti G
Gordiyenko Y
Santhanam B
Wolf J
Wiederhold K
Dornan GL
Skehel M
Robinson CV
Passmore LA
Architecture of eukaryotic mRNA 3′-end processing machinery
Science (New York, N.Y.)
Science
6366
358
1056
1059
2017
English
10.1126/science.aao6535
29074584
Raw movies and polished particles for PolyA polymerase module of the cleavage and polyadenylation factor (CPF) from Saccharomyces cerevisiae.
Polished shiny particles
/data/Particles
picked particles - multiframe - processed
MRCS
MRCS
459867
1
32 BIT FLOAT
128
variable
128
variable
This folder contains particles polished in RELION 3.1. The nominal pixel size is 1.40 A/px except for MRCS_Diamond folder, which is 1.36 A/px. The particles were pre-multiplied by CTF before cropping to counter the effect of signal delocalisation by CTF. The STAR file is not compatible with earlier versions of RELION.
Unaligned super-resolution movies
/data/Movies
micrographs - multiframe
MRCS
MRCS
3952
20
1
20
32 BIT FLOAT
7676
variable
7420
variable
This dataset was collected over five sessions.
Common parameters:
300 kV
Cs 2.7 mm
Nominal pixel sizes:
MRCS_Diamond: 0.68 Å / super-resolution pixel
Others: 0.70 Å / super-resolution pixel
Nominal dose rate:
MRCS_GAIN_1608: 2.24 e/A2/frame
MRCS_GAIN_1609: 2.0 e/A2/frame
MRCS_GAIN_1611: 2.23 e/A2/frame
MRCS_GO_161202: 1.8 e/A2/frame
MRCS_Diamond: 2.24 e/A2/frame