Cryo-electron microscopy structure of the P-Rex1–G-beta-gamma signaling scaffold

REL 2019-06-18 2019-11-19 2019-11-19 Cryo-electron microscopy structure of the P-Rex1–G-beta-gamma signaling scaffold 0000-0002-2067-4999 Michael Cianfrocco Department of Biological Chemistry, Life Sciences Institute, University of Michigan 210 Washtenaw Ave Ann Arbor MI United States 48109 0000-0002-2067-4999 Michael Cianfrocco Department of Biological Chemistry, Life Sciences Institute, University of Michigan 210 Washtenaw Ave Ann Arbor MI United States 48109 Cash JN Urata S Li S Ravala SK Avramova LV Shost MD Gutkind JS Tesmer JJG Cianfrocco MA 3.0 10.6019/EMPIAR-10285 EMDB EMD-20308 Cash JN Urata S Li S Ravala SK Avramova LV Shost MD Gutkind JS Tesmer JJG Cianfrocco MA Cryo-electron microscopy structure and analysis of the P-Rex1-Gβγ signaling scaffold Science advances Sci Adv 10 5 2019 English 10.1126/sciadv.aax8855 31663027

Raw movies (*.tif), micrographs (averages/*.mrc), particle coordinates (matching/*.star), and extracted stack (particles/*.mrcs) for P-Rex-Gbg cryo-EM structure. All data with prefix "18jan18c" were collected on an untilted grid whereas data with the prefix "18aug03b" were collected on a grid with a tilt angle of 30 degrees. Uploaded data were preprocessed using Warp-1.0.5. Original particle stack (905,464 particles): merge_18aug03b_18jan18c_goodparticles_Retrain1_BoxNet2Mask_20180531_2.star Selected stack after 2D classification (611,231 particles): cryosparc_exp001001_202.star Final stack after 3D heterogeneous refinement (205,599 particles): cryosparc_exp001054_009.star Warp re-trained model: Retrain1_BoxNet2Mask_20180531_2/ Gain reference for 18jan18c: 18jan18c_gain_reference_17133354_01_7420x7676_norm_0.mrc Gain reference for 18aug03b: 18aug03b_gain_reference_02131216_05_3710x3838_norm_0.mrc Kev=300 Cs=2.7 Pixel size=1A/pix Untilted (18jan18c) total dose: 49.5 e/A2 (30 frames) Tilted (18aug03b) total dose: 45 e/A2 (100 frames)

Movie files (.tif) for P-Rex1-Gbg /data/movies micrographs - multiframe MRCS TIFF 6746 30 UNSIGNED BYTE variable variable variable variable

Micrograph files (.mrc) & CTF log files for P-Rex1-Gbg /data/average micrographs - single frame MRC MRC 6736 1 32 BIT FLOAT variable variable variable variable

Extracted particles from Warp for P-Rex1-Gbg /data/particles picked particles - single frame - processed MRCS MRCS 6360 1 32 BIT FLOAT variable variable variable variable

Particle coordinate files (*.star) for P-Rex1-Gbg can be found in the directory data/matching

data/movies/*.tif data/particles/*.mrcs data/particles