<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10284" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2019-05-29</depositionDate>
            <releaseDate>2019-06-19</releaseDate>
            <updateDate>2019-07-25</updateDate>
        </keyDates>
        <title>Extracellular albumin and endosomal ions prime enterovirus particles for uncoating that can be prevented by fatty acid saturation</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-4592-5926</authorORCID>
            <firstName>Varpu</firstName>
            <lastName>Marjomäki</lastName>
            <organization type="academic">Department of Biological and Environmental Science, Nanoscience Center, University of Jyväskylä</organization>
            <street>Survontie 9</street>
            <townOrCity>Jyväskylä</townOrCity>
            <country>Finland</country>
            <postOrZipCode>40500</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-7060-5871</authorORCID>
            <firstName>Sarah</firstName>
            <middleName>Jane</middleName>
            <lastName>Butcher</lastName>
            <organization type="academic">Molecular and Integrative Biosciences Research Programme, Institute of Biotechnology, University of Helsinki</organization>
            <street>Viikinkaari 9</street>
            <townOrCity>Helsinki</townOrCity>
            <country>Finland</country>
            <postOrZipCode>00790</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-3772-6003">Domanska A</author>
            <author authorORCID="0000-0001-6805-0336">Ruokolainen VP</author>
            <author authorORCID="0000-0002-8541-520X">Pelliccia M</author>
            <author authorORCID="0000-0003-3981-280X">Laajala MA</author>
            <author authorORCID="0000-0002-4592-5926">Marjomäki VS</author>
            <author authorORCID="0000-0001-7060-5871">Butcher SJ</author>
        </authorsList>
        <datasetSize units="TB">2.4</datasetSize>
        <entryDOI>10.6019/EMPIAR-10284</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-4903</emdbEntry>
            <emdbEntry>EMD-0565</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0001-6805-0336" order="1">Ruokolainen VP</author>
                    <author authorORCID="0000-0002-3772-6003" order="2">Domanska A</author>
                    <author authorORCID="0000-0003-3981-280X" order="3">Laajala MA</author>
                    <author authorORCID="0000-0002-8541-520X" order="4">Pelliccia M</author>
                    <author authorORCID="0000-0001-7060-5871" order="5">Butcher SJ</author>
                    <author authorORCID="0000-0002-4592-5926" order="6">Marjomäki VS</author>
                    <title>Extracellular albumin and endosomal ions prime enterovirus particles for uncoating that can be prevented by fatty acid saturation</title>
                    <journal>Journal of virology</journal>
                    <journalAbbreviation>J. Virol.</journalAbbreviation>
                    <country></country>
                    <year>2019</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1128/jvi.00599-19</externalReferences>
                    <externalReferences type="pubmed">31189702</externalReferences>
                    <details>Uploaded micrographs are movies, each movie contains 30 frames with an accumulated dose of 30 e/Å2;  calibrated pixel size is 1.24 Å.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned multi-frame micrographs of control Echovirus 1</name>
        <directory>/data/E1_control/Marjomaki_echo_cntrl_fr120k_U6_1stg_20171120_132145</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>504</numImagesOrTiltSeries>
        <framesPerImage>30</framesPerImage>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>variable</imageWidth>
            <pixelWidth>1.24</pixelWidth>
            <imageHeight>variable</imageHeight>
            <pixelHeight>1.24</pixelHeight>
        </dimensions>
        <details></details>
        <segmentationList/>
        <micrographsFilePattern>data/E1_control/Marjomaki_echo_cntrl_fr120k_U6_1stg_20171120_132145/Images-Disc1/GridSquare_*/Data/*.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern>data/E1_control/coords_suffix_autopick.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/E1_control</pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned multi-frame micrographs of control Echovirus 1</name>
        <directory>/data/E1_control_2/Marjomaki_echo_cntrl_fr120k_U6_2ndg_20171121_102801</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>847</numImagesOrTiltSeries>
        <framesPerImage>30</framesPerImage>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>variable</imageWidth>
            <pixelWidth>1.24</pixelWidth>
            <imageHeight>variable</imageHeight>
            <pixelHeight>1.24</pixelHeight>
        </dimensions>
        <details>Two datasets of control EV1 were combined for processing.</details>
        <segmentationList/>
        <micrographsFilePattern>data/E1_control_2/Marjomaki_echo_cntrl_fr120k_U6_2ndg_20171121_102801/Images-Disc1/GridSquare_*/Data/*.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern>data/E1_control/coords_suffix_autopick.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/E1_control</pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unaligned multi-frame micrographs of treated Echovirus 1 (expanded particle)</name>
        <directory>/data/E1_treated/Marjomaki_echo_treated_fr120k_A37_1stg_20171127_121153</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>1246</numImagesOrTiltSeries>
        <framesPerImage>30</framesPerImage>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>variable</imageWidth>
            <pixelWidth>1.24</pixelWidth>
            <imageHeight>variable</imageHeight>
            <pixelHeight>1.24</pixelHeight>
        </dimensions>
        <details></details>
        <segmentationList/>
        <micrographsFilePattern>data/E1_treated/Marjomaki_echo_treated_fr120k_A37_1stg_20171127_121153/Images-Disc1/GridSquare_*/Data/*.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern>data/E1_treated/coords_suffix_autopick.star</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/E1_treated</pickedParticlesDirectory>
    </imageSet>
</entry>
