REL
2019-03-24
2019-07-25
2019-08-16
LAT1-CD98hc bound to MEM-108 Fab
0000-0003-1813-7008
Osamu
Nureki
Department of Biological Sciences, Graduate School of Science, The University of Tokyo
7-3-1 Hongo, Bunkyo-ku
Tokyo
Japan
113-0033
0000-0003-1813-7008
Osamu
Nureki
Department of Biological Sciences, Graduate School of Science, The University of Tokyo
7-3-1 Hongo, Bunkyo-ku
Tokyo
Japan
113-0033
Lee Y
Wiriyasermkul P
Jin C
Quan L
Ohgaki R
Okuda S
Kusakizako T
Nishizawa T
Oda K
Ishitani R
Yokoyama T
Nakane T
Shirouzu M
Endou H
Nagamori S
Kanai Y
Nureki O
4.1
10.6019/EMPIAR-10264
EMDB
EMD-9849
Lee Y
Wiriyasermkul P
Jin C
Quan L
Ohgaki R
Okuda S
Kusakizako T
Nishizawa T
Oda K
Ishitani R
Yokoyama T
Nakane T
Shirouzu M
Endou H
Nagamori S
Kanai Y
Nureki O
Quan L
Cryo-EM structure of the human L-type amino acid transporter 1 in complex with glycoprotein CD98hc
Nature structural & molecular biology
Nat. Struct. Mol. Biol.
2019
English
10.1038/s41594-019-0237-7
31160781
Non-gain-corrected micrograph movies in tiff format. Gain references must be rotated and flipped. In MotionCor2, this is "-RotGain 1 -FlipGain 2".
Exposure time: 15.6 seconds
Frame: 46 frames
Accumulated exposure: 50.2 e−/A2
Electron flux rate: 7.14 e−/pix/s
Unaligned multi-frame micrographs, Dataset1
/data/Dataset1
micrographs - multiframe
TIFF
TIFF
1582
60
1
60
SIGNED 16 BIT INTEGER
4096
0.8346
4096
0.8346
Unaligned, gain corrected movies from Falcon 3 in the counting mode. The MRC files from EPU were converted to the TIFF format by mrc2tif in IMOD. During this process, the pixel size in the image header became 1.0. The calibrated pixel size is 0.8346. The total exposure is 46.0 electrons per squared Angstom in a 46.5 second exposure. The data contain a few dozen micrographs that were excluded during the processing due to poor maximal CTF resolution.
Unaligned multi-frame micrographs, Dataset2
/data/Dataset2
micrographs - multiframe
TIFF
TIFF
1900
60
1
60
SIGNED 16 BIT INTEGER
4096
0.8346
4096
0.8346
Unaligned, gain corrected movies from Falcon 3 in the counting mode. The MRC files from EPU were converted to the TIFF format by mrc2tif in IMOD. During this process, the pixel size in the image header became 1.0. The calibrated pixel size is 0.8346. The total exposure is 46.0 electrons per squared Angstom in a 46.5 second exposure. The data contain a few dozen micrographs that were excluded during the processing due to poor maximal CTF resolution.
Micelle subtracted particles used for final reconstruction
/data/Particles
picked particles - single frame - processed
MRCS
MRCS
361967
1
32 BIT FLOAT
288
1.333
288
1.333
Micelle subtracted particles used for final reconstruction. The MRCS file contains 361,967 particles but some were rejected during classification. The final set of particles and the orientations are stored in the associated STAR file. The image header contains a wrong pixel size information (1.375 A) because of the assumed nominal pixel size (0.861 A). The actual calibrated pixel size is 1.333 A (0.8346 A x 460 pix / 288 pix due to down-sampling), which is used for the final reconstruction in the associated publication.