General microscopy settings: Acceleration voltage = 300 kV Cs = 2.7 mm Nominal pixel size = 1.34 A/px Total exposure = 40 e- / A^2 distributed over 40 frames Calibrated pixel size for postprocessing = 1.3 A/px Data structure: cfg/ - movie parameter data from FOCUS mrc/ - aligned averages from MotionCorr picking/particles_coordinate_master.txt - picking coordinates of all 231,688 particles in .box convention and including defocus information at the center of particle box stack/ - FREALIGN parameter files for the particle stack, at the beginning (particles_1_r1.par) and at the end (particles_9_r1.par) of the consensus refinement particles.mrcs - stack file containing 231,688 particles used in consensus refinement, 320 x 320 pixels