<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10233" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2018-12-11</depositionDate>
            <releaseDate>2019-05-01</releaseDate>
            <updateDate>2019-05-01</updateDate>
        </keyDates>
        <title>Retrieving High-Resolution Information from Disordered 2D Crystals by Single Particle Cryo-EM</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-1185-4592</authorORCID>
            <firstName>Henning</firstName>
            <lastName>Stahlberg</lastName>
            <organization type="academic">C-CINA, Biozentrum, University of Basel</organization>
            <street>Mattenstrasse 26</street>
            <townOrCity>Basel</townOrCity>
            <country>Switzerland</country>
            <postOrZipCode>4058</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-1185-4592</authorORCID>
            <firstName>Henning</firstName>
            <lastName>Stahlberg</lastName>
            <organization type="academic">C-CINA, Biozentrum, University of Basel</organization>
            <street>Mattenstrasse 26</street>
            <townOrCity>Basel</townOrCity>
            <country>Switzerland</country>
            <postOrZipCode>4058</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-4247-4303">Righetto R</author>
            <author authorORCID="0000-0002-5405-7112">Biyani N</author>
            <author authorORCID="0000-0003-4694-1896">Kowal J</author>
            <author authorORCID="0000-0002-8733-055X">Chami M</author>
            <author authorORCID="0000-0002-1185-4592">Stahlberg H</author>
        </authorsList>
        <datasetSize units="GB">103.0</datasetSize>
        <entryDOI>10.6019/EMPIAR-10233</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-4432</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0003-4247-4303" order="1">Righetto R</author>
                    <author authorORCID="0000-0002-5405-7112" order="2">Biyani N</author>
                    <author authorORCID="0000-0003-4694-1896" order="3">Kowal J</author>
                    <author authorORCID="0000-0002-8733-055X" order="4">Chami M</author>
                    <author authorORCID="0000-0002-1185-4592" order="5">Stahlberg H</author>
                    <title>Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM</title>
                    <journal>Nature communications</journal>
                    <journalAbbreviation>Nat Commun</journalAbbreviation>
                    <country></country>
                    <issue>1</issue>
                    <volume>10</volume>
                    <year>2019</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41467-019-09661-5</externalReferences>
                    <externalReferences type="pubmed">30979902</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Aligned averages of MloK1 2D crystals in the presence of cAMP</name>
        <directory>/data/mrc</directory>
        <category>micrographs - single frame</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>270</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>3808</imageWidth>
            <pixelWidth>1.34</pixelWidth>
            <imageHeight>3808</imageHeight>
            <pixelHeight>1.34</pixelHeight>
        </dimensions>
        <details>Micrographs are MotionCorr-aligned averages of 40 e-/A^2 exposures distributed across 40 movie frames using the Gatan K2 direct electron detector with energy filter.
Particle picking coordinates as determined by the conventional unbending algorithm are available in the file picking/particles_coordinate_master.txt</details>
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        <micrographsFilePattern>data/mrc/*.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern>data/picking/*.txt</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/picking</pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Particle stack from MloK1 2D crystals in the presence of cAMP</name>
        <directory>/data</directory>
        <category>picked particles - single frame - unprocessed</category>
        <headerFormat>MRCS</headerFormat>
        <dataFormat>MRCS</dataFormat>
        <numImagesOrTiltSeries>231688</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>320</imageWidth>
            <pixelWidth>1.34</pixelWidth>
            <imageHeight>320</imageHeight>
            <pixelHeight>1.34</pixelHeight>
        </dimensions>
        <details>Particle stack containing 231,688 patches of MloK1 2D crystals. Each particle box comprises roughly 3x3 MloK1 tetramers.</details>
        <segmentationList/>
        <micrographsFilePattern>data/mrc/*.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern>data/picking/*.txt</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/picking</pickedParticlesDirectory>
    </imageSet>
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