REL 2018-11-13 2018-11-23 2018-11-23 0000-0002-8124-7472 Dmitry Lyumkis The Salk Institute for Biological Studies 10010 North Torrey Pines Road La Jolla California United States 92037 0000-0002-8124-7472 Dmitry Lyumkis Laboratory of Genetics and Helmsley Center for Genomic Medicine, The Salk Institute for Biological Studies 10010 North Torrey Pines Road La Jolla California United States 92037 Zhang C Lyumkis D 66.9 10.6019/EMPIAR-10229 EMDB EMD-9015 Zhang C Konermann S Brideau N Lotfy P Wu X Novick SJ Strutzenberg T Griffin P Hsu PD Lyumkis D Cell Cell 1 175 212 223 2018 English 10.1016/j.cell.2018.09.001 30241607

Two datasets are deposited. The first dataset is a stack and associated .star file of 154,889 particles. This stack was used as input to common-lines-based ab initio 3D orientation assignment and reconstruction in cryoSparc. The procedure resulted in the automated selection of 15,846 particles, from which a reconstruction was performed. This reconstruction is reported in the associated publication and in EMD-9015. The second dataset is a stack and associated .star file that is generated from the selected 15,846 particles. Using these as input to ab initio orientation assignment and reconstruction in cryoSparc results in a similar map as the first dataset.

stack of ~150k particles used for ab initio orientation assignment and reconstruction /data/All_Particles picked particles - single frame - unprocessed MRCS MRCS 154889 1 32 BIT FLOAT 320 0.73 320 0.73

This directory contains a stack and associated .star file of 154,889 particles. This stack was used as input to common-lines-based ab initio 3D orientation assignment and reconstruction in cryoSparc. The procedure resulted in the automated selection of 15,846 particles, from which a reconstruction was performed. This reconstruction is reported in the associated publication and in EMD-9015.

stack of ~16k best particles from above /data/Best_Particles picked particles - single frame - processed MRCS MRCS 15846 1 32 BIT FLOAT 256 0.73 256 0.73

This directory contains a stack and associated .star file of the 15,846 best particles selected from the full stack. This stack represents the clean particles from above and reproduces the deposited map when subjected to an ab initio orientation assignment and reconstruction procedure.