REL 2018-08-02 2018-10-25 2022-02-08 0000-0003-2096-8310 David Bhella MRC - University of Glasgow Centre for Virus Research 464 Bearsden Road Glasgow Scotland United Kingdom G61 1QH 0000-0003-2096-8310 David Bhella MRC - University of Glasgow Centre for Virus Research 464 Bearsden Road Glasgow Scotland United Kingdom G61 1QH Ho KL Gabrielsen M Beh PL Kueh CL Thong QX Streetley J Tan WS Bhella D 130.4 10.6019/EMPIAR-10203 EMDB EMD-0129 Ho KL Gabrielsen M Beh PL Kueh CL Thong QX Streetley J Tan WS Bhella D PLoS biology PLoS Biol 10 16 2018 English 10.1371/journal.pbio.3000038 30346944 Motion corrected micrographs of Macrobrachium rosenbergii nodavirus virus-like particles /data micrographs - single frame MRC MRC 2459 1 32 BIT FLOAT 3838 1.06 3710 1.06

To collect high-resolution data on MrNV VLPs, grids were imaged at the electron bioimaging centre (eBIC), Diamond Light Source (UK) using an FEI Titan Krios operated at 47,170 X magnification. Images were recorded on a Gatan K2 BioQuantum energy-filtered direct detector camera operated in zero-loss imaging mode with a slit width of 20 eV. Five second exposures were recorded in electron counting mode, at a frame-rate of four frames per second and a dose rate of 1.8 electrons/pixel/frame. The pixel size was 1.06 Å/pixel. Motion correction was performed in Motioncor2.