<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10163" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2017-09-09</depositionDate>
            <releaseDate>2022-01-31</releaseDate>
            <updateDate>2022-02-01</updateDate>
        </keyDates>
        <title>Asymmetric structures of the uncleaved full-length HIV-1 envelope glycoprotein trimer</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-9302-2257</authorORCID>
            <firstName>Youdong</firstName>
            <middleName>Jack</middleName>
            <lastName>Mao</lastName>
            <organization type="academic">Dana-Farber Cancer Institute</organization>
            <street>450 Brookline Ave</street>
            <townOrCity>Boston</townOrCity>
            <stateOrProvince>MA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>02215</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-9302-2257</authorORCID>
            <firstName>Youdong</firstName>
            <middleName>Jack</middleName>
            <lastName>Mao</lastName>
            <organization type="academic">Dana-Farber Cancer Institute</organization>
            <street>450 Brookline Ave</street>
            <townOrCity>Boston</townOrCity>
            <stateOrProvince>MA</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>02215</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author>Zhang S</author>
            <author>Wang KY</author>
            <author>Wang WL</author>
            <author>Chen S</author>
            <author>Sodroski JG</author>
            <author authorORCID="0000-0001-9302-2257">Mao Y</author>
        </authorsList>
        <datasetSize units="TB">4.0</datasetSize>
        <entryDOI>10.6019/EMPIAR-10163</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-23860</emdbEntry>
            <emdbEntry>EMD-23861</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="true">
                    <author order="1">Zhang S</author>
                    <author authorORCID="0000-0001-6308-1619" order="2">Wang K</author>
                    <author authorORCID="0000-0002-5064-8097" order="3">Wang WL</author>
                    <author order="4">Nguyen HT</author>
                    <author order="5">Chen S</author>
                    <author order="6">Lu M</author>
                    <author order="7">Go EP</author>
                    <author order="8">Ding H</author>
                    <author order="9">Steinbock RT</author>
                    <author order="10">Desaire H</author>
                    <author order="11">Kappes JC</author>
                    <author order="12">Sodroski J</author>
                    <author authorORCID="0000-0001-9302-2257" order="13">Mao Y</author>
                    <title>Asymmetric structures and conformational plasticity of the uncleaved full-length human immunodeficiency virus (HIV-1) envelope glycoprotein trimer</title>
                    <journal></journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country></country>
                    <year>2021</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1101/2021.03.28.437443</externalReferences>
                </journalCitation>
            </universalCitation>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author order="1">Zhang S</author>
                    <author order="2">Wang K</author>
                    <author order="3">Wang WL</author>
                    <author order="4">Nguyen HT</author>
                    <author order="5">Chen S</author>
                    <author authorORCID="0000-0002-8099-6447" order="6">Lu M</author>
                    <author order="7">Go EP</author>
                    <author order="8">Ding H</author>
                    <author order="9">Steinbock RT</author>
                    <author order="10">Desaire H</author>
                    <author order="11">Kappes JC</author>
                    <author authorORCID="0000-0002-9750-1615" order="12">Sodroski J</author>
                    <author authorORCID="0000-0001-9302-2257" order="13">Mao Y</author>
                    <title>Asymmetric structures and conformational plasticity of the uncleaved full-length human immunodeficiency virus (HIV-1) envelope glycoprotein trimer</title>
                    <journal>Journal of virology</journal>
                    <journalAbbreviation>J Virol</journalAbbreviation>
                    <country></country>
                    <year>2021</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1128/jvi.00529-21</externalReferences>
                    <externalReferences type="pubmed">34549974</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Drift-corrected micrographs of the uncleaved full-length HIV-1 envelope glycoprotein trimer (300 kV)</name>
        <directory>/data/mic_300kV</directory>
        <category>micrographs - single frame</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>8031</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>7676</imageWidth>
            <pixelWidth>0.685</pixelWidth>
            <imageHeight>7420</imageHeight>
            <pixelHeight>0.685</pixelHeight>
        </dimensions>
        <details>The micrographs were the drift-corrected averages of 38 raw movie frames. Each original movie was recored in 40 frames accumulating 53 electron per squared angstrom in 10 second exposure, with the first two frame not being used after drift correction for the averaged micrographs.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/particles_300kV</pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Drift-corrected micrographs of the uncleaved full-length HIV-1 envelope glycoprotein trimer (200 kV)</name>
        <directory>/data/mic_200kV</directory>
        <category>micrographs - single frame</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>10812</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>7420</imageWidth>
            <pixelWidth>0.76</pixelWidth>
            <imageHeight>7676</imageHeight>
            <pixelHeight>0.76</pixelHeight>
        </dimensions>
        <details></details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/particles_200kV</pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>The classified particle stacks of the full-length HIV-1 envelope precursor in the P2 class</name>
        <directory>/data/mic_TiltPair</directory>
        <category>picked particles - single frame - processed</category>
        <headerFormat>XML</headerFormat>
        <dataFormat>BIG DATA VIEWER HDF5</dataFormat>
        <numImagesOrTiltSeries>51</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>7420</imageWidth>
            <pixelWidth>0.76</pixelWidth>
            <imageHeight>7676</imageHeight>
            <pixelHeight>0.76</pixelHeight>
        </dimensions>
        <details>The picked particles were binned twice to 140x140 box size and a pixel size of 1.52 angstrom.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/particles_TiltPair_P1</pickedParticlesDirectory>
    </imageSet>
</entry>
