REL 2018-02-21 2018-05-02 2018-05-02 0000-0002-2778-3936 Wanda Kukulski MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Cambridgeshire United Kingdom CB2 0QH 0000-0002-2778-3936 Wanda Kukulski MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge Cambridgeshire United Kingdom CB2 0QH Bharat TAM Hoffmann PC Kukulski W 27.9 10.6019/EMPIAR-10161 EMDB EMD-4305 Bharat TAM Hoffmann PC Kukulski W Structure (London, England : 1993) Structure 2018 English 10.1016/j.str.2018.03.015 29681471

Each folder contains one raw tilt series (ordered by tilt angle) and the relevant correlative microscopy files. These are: *_GFP_original.tif: raw GFP image (obtained by cryo-FM) containing the signal imaged by the tilt series *_em_map_original.tif: medium magnification EM image used to locate the signal in the EM *_GFP_tranformed_to_em_map: the GFP image, transformed based on fiducial markers, to register onto the EM image *_em_map_annotated.jpg: annotated version of the medium magnification EM image, in which the predicted position of the GFP spots of interest (pink) and the fiducial markers used for the transformation are indicated (blue: selected positions; green: predicted positions). Tranformations are done using MATLAB-based scripts published in Kukulski et al., JCB 2011.

9 folders each containing raw tilt series of vitreous sections of yeast cells and the corresponding correlative microscopy data /data/empiar tilt series MRC MRC 9 1 UNSIGNED 16 BIT INTEGER 3704 3.5 3832 3.5

Each folder contains one tilt series and a subfolder with correlative micrscopy data that was used to identify siganls of the eisosome protein Pil1-GFP within the tilt series.