<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10158" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2018-02-16</depositionDate>
            <releaseDate>2018-04-27</releaseDate>
            <updateDate>2021-05-28</updateDate>
        </keyDates>
        <title>Cryo electron tomography of immotile sea urchin sperm flagella</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-0122-7173</authorORCID>
            <firstName>Daniela</firstName>
            <lastName>Nicastro</lastName>
            <organization type="academic">Departments of Cell Biology and Biophysics, University of Texas Southwestern Medical Center, Dallas</organization>
            <street>NL5-110A, 6000 Harry Hines Blv</street>
            <townOrCity>Dallas</townOrCity>
            <stateOrProvince>Texas</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>75235</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-0122-7173</authorORCID>
            <firstName>Daniela</firstName>
            <lastName>Nicastro</lastName>
            <organization type="academic">Departments of Cell Biology and Biophysics, University of Texas Southwestern Medical Center, Dallas</organization>
            <street>NL5-110A, 6000 Harry Hines Blv</street>
            <townOrCity>Dallas</townOrCity>
            <stateOrProvince>Texas</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>75235</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0001-5732-1209">Lin J</author>
            <author authorORCID="0000-0002-0122-7173">Nicastro D</author>
        </authorsList>
        <datasetSize units="GB">13.8</datasetSize>
        <entryDOI>10.6019/EMPIAR-10158</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-8835</emdbEntry>
            <emdbEntry>EMD-8837</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0001-5732-1209" order="1">Lin J</author>
                    <author authorORCID="0000-0002-0122-7173" order="2">Nicastro D</author>
                    <title>Asymmetric distribution and spatial switching of dynein activity generates ciliary motility</title>
                    <journal>Science (New York, N.Y.)</journal>
                    <journalAbbreviation>Science</journalAbbreviation>
                    <country></country>
                    <issue>6387</issue>
                    <volume>360</volume>
                    <year>2018</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1126/science.aar1968</externalReferences>
                    <externalReferences type="pubmed">29700238</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
        <relatedEmpiarEntries>
            <empiarEntry>EMPIAR-10157</empiarEntry>
        </relatedEmpiarEntries>
    </crossReferences>
    <imageSet>
        <name>Tilt series of immotile sea urchin sperm flagella</name>
        <directory>/data</directory>
        <category>tilt series</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>27</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>2048</imageWidth>
            <pixelWidth>9.856</pixelWidth>
            <imageHeight>2048</imageHeight>
            <pixelHeight>9.856</pixelHeight>
        </dimensions>
        <details>These 27 tilt series were collected from two different types of control samples using a FEI Tecnai F30 electron microscope and were control data of EMPIAR-10157. A sub-tomogram average derived from the first 20 tilt series (S1-S20) of EHNA inhibited immotile sea urchin sperm flagella has been deposited in EMDB under ID code EMD-8835;  A sub-tomogram average derived from the last 7 tilt series (S21-S27) of the other control sample (Triton X-100  demembranated sea urchin flagella) has been deposited in EMDB under ID code EMD-8837.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
