REL
2017-06-27
2018-02-07
2018-02-07
Raw 2d tomographic tilt series of a dividing cell
0000-0001-5909-701X
Jan
Ellenberg
European Molecular Biology Laboratory
Meyerhofstrasse 1
Heidelberg
Germany
69117
0000-0001-5909-701X
Jan
Ellenberg
European Molecular Biology Laboratory
Meyerhofstrasse 1
Heidelberg
Germany
69117
Otsuka S
245.4
10.6019/EMPIAR-10116
EMDB
EMD-3820
Otsuka S
Steyer AM
Schorb M
Hériché JK
Hossain MJ
Sethi S
Kueblbeck M
Schwab Y
Beck M
Ellenberg J
Postmitotic nuclear pore assembly proceeds by radial dilation of small membrane openings
Nature structural & molecular biology
Nat. Struct. Mol. Biol.
1
25
21
28
2017
English
10.1038/s41594-017-0001-9
29323269
Images were recorded over a -60° to 60° tilt range with an angular increment 1° at a pixel size of 0.75 nm.
Raw 2D tilt series for all the electron tomograms
/data
tilt series
ST
ST
65
121
1
121
SIGNED 16 BIT INTEGER
4096
7.5
4096
7.5
HeLa cells were instantly frozen using a high-pressure freezing machine (HPM 010; ABRA Fluid AG, Widnau, Switzerland) and freeze-substituted into Lowicryl HM20 resin (Polysciences Inc., Warrington, PA). As fiducial markers, 15 nm of gold-conjugated Protein A (CMC university Medical Center Utrecht, Utrecht, Netherlands) was absorbed on both sides of 300 nm sections. Sections were post-stained with 2% UA and lead citrate. Single or dual axis tilt series were acquired with a TECNAI TF30 trans-mission EM (TEM; 300 kV; FEI, Hillsboro, OR) equipped with a 4k x 4k Eagle camera (FEI) by using the Serial EM software. Images were recorded over a -60° to 60° tilt range with an angular increment 1°.