REL
2016-11-03
2016-11-10
2016-11-10
Volta phase plate with defocus cryo-EM dataset of Thermoplasma acidophilum 20S proteasome
0000-0001-6406-8993
Radostin
Danev
Max Planck Institute of Biochemistry
Am Klopferspitz 18
Martinsried
Germany
82152
0000-0001-6406-8993
Radostin
Danev
Max Planck Institute of Biochemistry
Am Klopferspitz 18
Martinsried
Germany
82152
Danev R
Tegunov D
Baumeister W
1
2017-01-03
REL
Added the citation information.
92.5
10.6019/EMPIAR-10078
EMDB
EMD-3455
EMD-3456
EMD-3457
Danev R
Tegunov D
Baumeister W
Using the Volta phase plate with defocus for cryo-EM single particle analysis
bioRxiv
bioRxiv
1
10.1101/085530
http://biorxiv.org/content/early/2016/11/03/085530
Unaligned multi-frame micrographs of 20S proteasome acquired with a Volta phase plate and defocus
/data
micrographs - multiframe
TIFF
TIFF
427
24
UNSIGNED BYTE
7676
0.53
7420
0.53
The movies were acquired with SerialEM as dark-subtracted but non-gain-normalized stacks and saved as LZW-compressed TIFFs. The gain reference is in the "SuperRef_20S_000_Mar28_14.49.23.mrc" file. "MotionCor2_patch9_DW1.sh" is an example shell script for using MotionCor2 to gain normalize, align and dose-weight the movies. "micrographs_ctf.star" is a Relion 2.0 .star file containing the CTF fit results from ctffind4 v4.1.5. "particles.star" is a Relion 2.0 .star file containing particle coordinates and CTF fit parameters. Other details about the dataset are in "dataset_parameters.txt".
The data was used for three reconstruction strategies. All frames were used in the Relion particle polishing and Warp approaches. The first two frames were discarded in the MotionCor2 approach.