REL 2016-10-31 2016-12-02 2016-12-02 0000-0001-6656-6320 Yong Zi Tan New York Structural Biology Center/Columbia University 89 Convent Ave New York New York United States 10027 0000-0002-8124-7472 Dmitry Lyumkis Laboratory of Genetics and Helmsley Center for Genomic Medicine, The Salk Institute for Biological Studies 10010 North Torrey Pines Road La Jolla California United States 92037 0000-0002-8772-468X Williamson R James Department of Integrative Structural and Computational Biology, Department of Chemistry, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute 10550 North Torrey Pines Road La Jolla California United States 92037 Davis JH Tan YZ Carragher B Potter CS Lyumkis D Williamson JR 50.3 10.6019/EMPIAR-10076 EMDB EMD-8434 EMD-8440 EMD-8441 EMD-8442 EMD-8443 EMD-8444 EMD-8445 EMD-8446 EMD-8447 EMD-8448 EMD-8449 EMD-8450 EMD-8451 EMD-8452 EMD-8453 EMD-8455 EMD-8456 EMD-8457 Davis JH Tan Y Carragher B Potter CS Lyumkis D Williamson JR Cell Cell 6 167 1610 1622.e15 2016 English 10.1016/j.cell.2016.11.020

131899 particle stack, aligned on a per-particle basis using Rubinstein's particle polishing software, exposure-weighted, not inverted, normalized. Stack contains a heterogeneous mixture of particles primarily comprising assembling 50S ribosomal subunits.

Particle stack of L17-depleted 50S Ribosomal Assembly Intermediates /data picked particles - multiframe - unprocessed MRC MRC 131899 1 1 1 32 BIT FLOAT 320 1.31 320 1.31

131899 particle stack has been obtained from 2479 images with 50 frames per image, using frames from 1 to 50, aligned on a per-particle basis using Rubinstein's particle polishing software, exposure-weighted, not inverted, normalized. Stack contains a heterogeneous mixture of particles. In order to account for highly preferred orientation of the specimen, data was acquired using tilts ranging from 10-60 degrees, in addition to untilted images at 0 degrees. Parameter file for Frealign and Relion (stack is not yet normalized to Relion format) provided.